Development of a procedure for standardization of the Rozmatin substance from Moldavian dragonhead (Dracocephalum moldavice) herb
- Authors: Zvezdina E.V.1, Sheichenko O.P.1, Burova A.E.1
-
Affiliations:
- All-Russian Research Institute of Medicinal and Aromatic Plants
- Issue: Vol 69, No 6 (2020)
- Pages: 25-30
- Section: Articles
- URL: https://journals.eco-vector.com/0367-3014/article/view/113371
- DOI: https://doi.org/10.29296/25419218-2020-06-05
- ID: 113371
Cite item
Open Access
Access granted
Abstract
Moldavian dragonhead (Dracocephalum moldavica) is an annual herbaceous plant that is widely cultivated in Eastern and Central Europe as an essential oil-bearing, melliferous, and spicy-and-aromatic plant. The All-Russian Research Institute of Medicinal and Aromatic Plants has developed a technology for obtaining a dry purified Moldavian dragonhead herb extract arbitrarily called Rozmatin that contains flavonoids and phenylpropanoids. The substance has a tonic effect on the cardiovascular and nervous systems, as well as an anti-inflammatory effect. Objective: to develop a procedure for standardizing the Rozmatin substance and to make its validation assessment. Material and methods. The Rozmatin substance obtained from the aboveground part of the Moldavian dragonhead (the variety «Tenderness») growing in the All-Russian Research Institute of Medicinal and Aromatic Plants, which was harvested during the flowering phase. The investigators used the following techniques: thin layer chromatography (TLC), UV spectrophotometry, as well as the equipment: 10x15-cm Sorbfil ПТСХ-ПА plates (Russia) and UV-1800 (Shimadzu) and Cary 100 Scan (Varian) spectrophotometers. Results. The presence of flavonoids in the substance was confirmed by the cyanidin test (Shinoda's test) (a pink and red color). The UV spectrum of Rozmatin showed two absorption maxima at 327±2 and 288±2 nm in the wavelength range of 250 to 400 nm. The total phenolic compound content in the Rozmatin substance in terms of rosmarinic acid, which was determined using direct spectrophotometry, ranged from 60.67 to 88.02% in 5 series of balanced loads. Conclusion. Procedures have been developed for the qualitative and quantitative determination of phenolic compounds in the Rozmatin substance. The procedure for the quantitative determination of the total content phenolic compounds calculated with reference to rosmarinic acid meets all validation criteria.
Full Text
About the authors
Ekaterina Vladimirovna Zvezdina
All-Russian Research Institute of Medicinal and Aromatic Plants
Email: catterina@inbox.ru
Senior Researcher, Department of Phytochemistry and Standardization, postgraduate student
Olga Petrovna Sheichenko
All-Russian Research Institute of Medicinal and Aromatic Plants
Email: vilarnii.sheychenko@mail.ru
Leading Researcher, Department of Phytochemistry and Standardization
Alla Evgen'evna Burova
All-Russian Research Institute of Medicinal and Aromatic Plants
Email: alla.burova@inbox.ru
Senior Researcher, Department of Phytochemistry and Standardization
References
- Попова О.И., Никитина А. С. Змееголовник молдавский и иссоп лекарственный: современный взгляд на растения: монография. Волгоград; Издательство ВолгГМУ, 2014; 224.
- Тоцкая С.А., Грязнов М.Ю. Некоторые особенности выращивания нового сорта змееголовника молдавского селекции ФГБНУ ВИЛАР. Вопросы биологической, медицинской и фармацевтической химии. 2018; 9: 43-7.
- Jiang J., Yuan X., Wang T., Chen H., Zhao H., Yan X., Wang Z., Sun X., Qiusheng Z. Antioxidative and Cardioprotective Effects of Total Flavonoids Extracted from Dracocephalum moldavica L. Against Acute Ischemia/Reperfusion-Induced Myocardial Injury in Isolated Rat Heart. Cardiovascular Toxicology. 2014; 14: 74-82.
- Zeng Q., Jin H.Z., Qin J.J., Fu J.J., Hu X.J., Lin J.H., Yan L., Chen M., Zhang W.D. Chemical constituents of plants from the genus Dracocephalum. Chem. Biodivers. 2010; 7: 1911-29.
- Martinez-Vazquez M., Estrada-Reyes R., Martinez-Laurrabaquio A., Lopez-Rubalcavac C., Heinze G. Neuropharmacological study of Dracocephalum moldavica L. (Lamiaceae) in mice: Sedative effect and chemical analysis of an aqueous extract. J. of Ethnopharmacology. 2012; 141: 908-17.
- Yang S., Wang L., Guo X., Lou H., Ren D. A new flavonoid glycoside and other constituents from Dracocephalum moldavica. Natural Product Research. 2013; 27 (3): 201-7.
- Yang L.N., Xing J.G., He C.H., Wu T. The phenolic compounds from Dracocephalum moldavica L. Biochemical Systematics and Ecology. 2014; 54: 19-22.
- Yang L.N., Xing J.G., He C.H., Yang P.M. Chemical constituents of Dracocephalum Moldavica L. and their pharmacological activities. World Clin. Drugs. 2013; 34: 57.
- Dastmalchi K., Damien Dorman H.J., Muberra K., Hiltunen R. Chemical composition and in vitro antioxidant evaluation of a watersoluble Moldavian balm (Dracocephalum moldavica L.) extract. LWT Food Sci Technol. 2007; 40: 239-48.
- Zuniga M.I.J., Mariles A.J.H., Flores J.L.C., Herrera J.A.M., Sotelo M.G.R., Montes G.I.C., Gomez y Gomez Y. de las M. AntidepressantLike Effects of Dracocephalum moldavica L. in Mouse Models of Immobility Tests. Pharmacognosy J. 2019; 11 (5): 976-83.
- Звездина Е.В., Курманова Е.Н., Ферубко Е.В. Противовоспалительная активность экстракта змееголовника молдавского под условным названием Розматин. Современные тенденции развития технологий здоровьесбережения: сборник трудов Седьмой научной конференции с международным участием. ФГБНУ ВИЛАР. М., 2019; 440-4.
- Панин В.П., Мартынчик И.А. Влияние змееголовника молдавского (Dracocephalum moldavica l.) на некоторые показатели центральной нервной системы мышей при курсовом введении. Современные тенденции развития технологий здоровьесбережения: сборник трудов Седьмой научной конференции с международным участием. ФГБНУ ВИЛАР. М., 2019; 473-8.
Supplementary files
