ELABORATION OF APPROACHES TO STANDARDIZING THE COMMON LILAC (SYRINGA VULGARIS) FLOWERS

Introduction. The representatives of the Syringa genus are valuable medicinal plants that have immunomodulatory, adaptogenic, antidepressant, and anxiolytic activities. The bark of common lilac (Syringa vulgaris) is registered as a medicinal plant raw material. The promising type of raw material is common lilac flowers that contain phenylpropanoids. Objective: to develop a procedure for quantitative determination of the amount of phenylpropanoids in the common lilac flowers. Material and methods. Common lilac flowers were investigated by direct spectrophotometry using the standard sample of chlorogenic acid. Results. The investigators defined optimal conditions for the extraction of phenylpropanoids from common lilac flowers: the extraction solvent was 60% ethyl alcohol; the raw material-to-extraction solvent ratio was 1:100; the extraction time was 45 min; the grinding degree was 1 mm. The total content of phenylpropanoids calculated with reference to chlorogenic acid in the common lilac flowers was found to range from 5.54 to 6.19%. Conclusion. A procedure has been developed to quantify the amount of phenylpropanoids in the common lilac flowers by direct spectrophotometry using the standard sample of chlorogenic acid at an analytical wavelength of 330 nm. The error of a single measurement at a 95% confidence interval is ±1.89%.

Syringa vulgaris L, common lilac, Syringa vulgaris L, flowers, phenylpropanoids, standardization, spectrophotometry, chlorogenic acid