Development of anti-adhesion membrane technology for isolation of intestinal anastomosis

Introduction. According to statistics, the incidence of complications after operations on the abdominal organs in the form of peritoneal adhesions is 63–97%. Most often, PS are formed after surgical interventions on the intestine, resulting in the development of intestinal obstruction. This complication can lead to dehydration, electrolyte imbalance, necrosis, abdominal abscess, kidney failure, intestinal perforation, and sepsis. Currently, the lack of effective anti-adhesion barriers determines the relevance of the development of new domestic remedies for the prevention of the development of postoperative adhesions, especially during surgical interventions on the intestines.

Purpose of the study. Development of a technology for obtaining a biodegradable anti-adhesion membrane.

Material and methods. To obtain a biodegradable antiadhesion membrane, a mixture of biopolymer solutions of chitosan and gelatin in various ratios was used as film formers. Anti-adhesion membranes were obtained by Young's method. Biodegradation was studied in rat blood plasma and sodium phosphate buffer solution.

Results. In the course of the studies, it was found that the temperature and humidity at which drying is performed do not have a significant effect on the qualitative characteristics of the membranes, and the main factors that determine their structural and mechanical properties are the composition and thickness of the layer of the mixture of film formers. Thus, membranes based on film-forming solutions of gelatin and chitosan in ratios of 2.5:1.5 provide a tensile strength of 86.4 MPa, an elongation at break of 6.7%, a thickness of 34 μm, which is considered satisfactory indicators of the structural and mechanical properties of biodegradable antiadhesion agents. membranes.

Conclusion. A technology for obtaining a biodegradable anti-adhesion membrane based on biopolymers has been developed. In rat blood plasma, membrane degradation occurred much faster than in sodium phosphate buffer solution, which indicates enzymatic degradation of membranes.