Sorption technology for the isolation of pancreatic enzymes from extracts of the pancreas of cattle and reindeer
- Authors: Karavaeva L.I.1, Krylova P.A.1, Moroz A.S.2, Kotova N.V.1, Glazova N.V.1, Bunyatyan N.D.3
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Affiliations:
- St. Petersburg Chemical and Pharmaceutical University
- Peter the Great St. Petersburg Polytechnic University
- "Scientific Centre for Expert Evaluation of Medicinal Products" of the Ministry of Health of the Russian Federation
- Issue: Vol 72, No 5 (2023)
- Pages: 39-44
- Section: Technology of medicines
- URL: https://journals.eco-vector.com/0367-3014/article/view/568105
- DOI: https://doi.org/10.29296/25419218-2023-05-05
- ID: 568105
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Abstract
Introduction. Pancreatin is a digestive enzyme remedy that is an extract of a pancreatic enzyme. It contains such proteolytic enzymes as amylase, lipase and protease, which are involved in the digestion of carbohydrates, fats, proteins, which contributes to their more complete absorption in the small intestine. It is used for insufficiency of the exocrine function of the pancreas (chronic pancreatitis, cystic fibrosis), for chronic inflammatory and degenerative diseases of the stomach, intestines, liver, gallbladder; conditions after resection or irradiation of these organs, accompanied by disorders of food digestion, flatulence, diarrhea (as part of combination therapy), to improve food digestion in patients with normal function of the gastrointestinal tract (GIT) in case of nutritional errors, as well as chewing disorders function, forced prolonged immobilization, sedentary lifestyle, and in preparation for x-ray and ultrasound examination of the abdominal organs.Thus, pancreatin is one of the most popular medical preparations and is produced under various trade names. However, pancreatin is not produced in Russia and is supplied to the country mainly from China and Germany.
Objective: Development of a technology for the isolation of pacreatic enzymes from extracts of the semigastric glands of SO and cattle.
Material and methods. In the work used – the pancreas from the reindeer. The determination of the component composition was carried out by the methods of gel chromatography.
Results. Methods have been developed for isolating a complex of digestive enzymes from the pancreas of CO and cattle.
Conclusion. The optimal conditions for the process of BAS extraction from the pancreas of CO and the pancreas of cattle have been selected. The component composition of the obtained extracts was determined.The optimization of the sorption technology for the isolation of enzymes from extracts of cattle and CO was developed and carried out.
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About the authors
Lolita I. Karavaeva
St. Petersburg Chemical and Pharmaceutical University
Author for correspondence.
Email: lolita.karavaeva@spcpu.ru
ORCID iD: 0000-0001-8960-4617
Post-graduate student of the Department of Biotechnology
Russian Federation, Professor Popov str., 14, Sant-Petersburg, 197022Polina A. Krylova
St. Petersburg Chemical and Pharmaceutical University
Email: polina.krylova@spcpu.ru
ORCID iD: 0000-0003-2532-248X
student of the Department of Biotechnology
Russian Federation, Professor Popov str., 14, Sant-Petersburg, 197022Anna S. Moroz
Peter the Great St. Petersburg Polytechnic University
Email: anna.kurochkina@pharminnotech.com
ORCID iD: 0009-0003-9536-7058
Assistant of the Department of Biotechnology
Russian Federation, Politechnicheskaya str., 29, Saint-Petersburg, 195251Natalia V. Kotova
St. Petersburg Chemical and Pharmaceutical University
Email: Natalia.kotova@pharminnotech.com
ORCID iD: 0000-0001-5483-6717
Associate Professor, Department of Biotechnology, Candidate of Chemical Sciences
Russian Federation, Professor Popov str., 14, Sant-Petersburg, 197022Natalya V. Glazova
St. Petersburg Chemical and Pharmaceutical University
Email: Natalia.glazova@pharminnotech.com
ORCID iD: 0000-0003-3242-3051
Associate Professor of the Department of Biotechnology, Candidate of Chemical Sciences
Russian Federation, Professor Popov str., 14, Sant-Petersburg, 197022Natalia D. Bunyatyan
"Scientific Centre for Expert Evaluation of Medicinal Products" of the Ministry of Health of the Russian Federation
Email: Bunyatyan@expmed.ru
ORCID iD: 0000-0003-0936-5551
Chief Analyst of the Center for Clinical Pharmacology, Doctor of Pharmaceutical Sciences, Professor
Russian Federation, Petrovsky boulevard, 8/2, Moscow, 127051References
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