Development of approaches to standardization of the herb of the meadowsweet

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Abstract

Introduction. Meadowseet Filipendula ulmaria (L.) Maxim. is widely distributed in the European part of Russia, in Western and Eastern Siberia. The plant has long been used in folk medicine, because it has a significant range of medicinal properties, many of which are associated with the presence of plant phenolic compounds, namely flavonoids.

The purpose of this study is to develop a technique for quantifying the total of flavonoids in the herb of the meadowsweet, including optimization of the flavonoid extraction process using methods of mathematical planning of the experiment and spectrophotometric analysis of the extracts obtained.

Material and methods. The material of the study was the herb of the meadowsweet, harvested in the period from 2020 to 2022 in the village of Alekseevka of the Samara region during the flowering phase (June–July).

Biologically active compounds were extracted from plant raw materials, varying the main parameters of the extraction process in accordance with the selected experimental plan, followed by determining the conditions for maximum output product.

The quantitative determination of the total of flavonoids in extracts from plant raw materials was carried out by differential spectrophotometry on the SF-2000 device (SDB Spectrum).

Results. Optimal conditions for the extraction of flavonoids from raw materials were established: extractant – 40% ethyl alcohol, the ratio of "raw material-extractant" – 1:50, extraction time – 90 minutes. A method of quantitative determination of the total of flavonoids calculated on standard sample of rutin in the herb of the meadowsweet by differential spectrophotometry has been developed. It was determined that the content of the total of flavonoids in the herb of the meadowsweet varies from 3.90±0.02% to 4.02±0.04%.

Conclusion. As a result of the conducted research, a technique for the quantitative determination of flavonoids in the herb of the meadowsweet has been developed, based on the optimization of the extraction process by the method of mathematical planning of the experiment and differential spectrophotometry of the extracts obtained.

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About the authors

Nikita Sergeevich Sokolov

Samara State Medical University

Author for correspondence.
Email: n.s.sokolov@samsmu.ru
ORCID iD: 0000-0001-8138-4306

Postgraduate Student of the Department of Chemistry of the Institute of Pharmacy, Samara State Medical University

Russian Federation, Chapaevskaya ul., 89, Samara, 443099

Vladimir Alexandrovich Kurkin

Samara State Medical University

Email: v.a.kurkin@samsmu.ru
ORCID iD: 0000-0002-7513-9352

Head of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy, Samara State Medical University, Doctor of Pharmaceutical Sciences, Professor

Russian Federation, Chapaevskaya ul., 89, Samara, 443099

Safia Khakimovna Sharipova

Samara State Medical University

Email: s.h.sharipova@samsmu.ru
ORCID iD: 0000-0002-6532-4749

Candidate of Chemical Sciences, Associate Professor, Department of Chemistry of the Institute of Pharmacy, Samara State Medical University

Russian Federation, Chapaevskaya ul., 89, Samara, 443099

Ksenia Nikolaevna Sazanova

Samara State Medical University

Email: k.n.sazanova@samsmu.ru
ORCID iD: 0000-0002-4287-8386

Candidate of Pharmaceutical Sciences, Senior Lecturer Department of Management and Economics of Pharmacy, Samara State Medical University

Russian Federation, Chapaevskaya ul., 89, Samara, 443099

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2. Fig. 1. UV spectrum of water-alcohol extraction from the herb of the meadowsweet (1) and water-alcohol extraction with aluminum chloride (2)

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3. Fig. 2. UV spectrum of the solution of water-alcohol extraction from the herb of the meadowsweet (differential version)

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