The study of the morphology and anatomy of leaves aronia Mitchurinii by various microscopic methods

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Abstract

Introduction. Aronia Mitchurina leaves (Aronia mitschurinii A.K. Skvortsov & Maitul) is a potentially perspective source of biologically active substances such as flavonoids, tannins, anthocyanins, etc. One of the phases of leaf standardization is the determination of the authenticity indicators. Previously, the main anatomical and diagnostic features of Aronia Mitchurina leaves were identified using the optical microscopy. For a more detailed study of the morphological and anatomical features of the leaf structure, the work used luminescence, scanning electron and stereomicroscopy.

Objective: To determine the morphological and anatomical structure of Michurina aronia leaves by luminescence, scanning electron and stereomicroscopy.

Material and methods. The object of the study was dried to a moisture content of not more than 10% Aronia Mitchurina leaves variety «Mulatka», harvested in the Tambov region during the technical maturity of fruits (August–September 2022). The study of morphological and anatomical features of leaves was performed according to the State Pharmacopoeia of the Russian Federation XIV, OFS.1.5.3.0003.15 «Technique of microscopic and microchemical study of medicinal plant raw materials and herbal medicinal preparations». For stereomicroscopic analysis used Biomed-2 microscope with lenses ×400, ×100 (Russia). Luminescence analysis was carried out with a Nikon ECLIPSE Ni-E/Ni-U microscope (Japan) in ultraviolet (UV) light. The scanning electron microscopy was performed using a JSM-6380LV JEOL (Japan) scanning electron microscope with an INCA 250 microanalysis system.

Results. The stereomicroscopic analysis showed that the main diagnostic features of Aronia Mitchurina leaves are long thin trichomes on the lower leaf surface and dark brown glands concentrated along the main vein of the upper surface of the leaf lamina. The characteristic fluorescence features of the studied raw material have been established. Luminescence is characteristic for conductive system and trichomes of the under surface of the leaf. Luminescence of glands and their bases is absent. The structure of stomatal apparatus, trichomes and glands was clarified by scanning microscopy.

Conclusion. For the first time, a comprehensive study of the main diagnostic features of Michurina aronia leaves by stereo-, luminescence and scanning electron microscopy was carried out. The structure and features of luminescence of morphological and anatomical structures of leaves were clarified. The carried out researches can be used for express identification of Michurina aronia leaves and will allow to prepare sections «External signs» and «Microscopic signs» for the draft pharmacopoeial article «Michurina aronia leaves».

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About the authors

Olga Valerievna Pugacheva

Voronezh State University

Author for correspondence.
Email: pugachevaov1@yandex.ru
ORCID iD: 0009-0003-9170-3130

Assistant Professor in the Department of Pharmaceutical Chemistry and Pharmaceutical Technology, Faculty of Pharmacy, Voronezh State University

Russian Federation, Universitetskaya pl., 1, Voronezh, 394018

Olga Valeryevna Trineeva

Voronezh State University

Email: trineevaov@mail.ru
ORCID iD: 0000-0002-1421-5067

PhD in Pharmaceutical Sciences, Associate Professor, Professor at the Department of Pharmaceutical Chemistry and Pharmaceutical Technology, Faculty of Pharmacy, Voronezh State University

Russian Federation, Universitetskaya pl., 1, Voronezh, 394018

References

  1. Kuklina A.G. Naturalisation Aronia Mitschurinii In The Forests Of European Russia. Lesokhozyaistvennaya informatsiya. 2015; 2: 46–56 (in Russian)
  2. Mark H.B., Bryan A.C., Lanfang H.L. et al. Anthocyanins, total phenolics, ORAC and moisture content of wild and cultivated dark-fruited Aronia species. Scientia Horticulturae. 2017; 224: 332–42. doi: 10.1016/j.scienta.2017.06.021 (in Russian)
  3. Deineka V.I., Tret'yakov M.Yu., Oleinits E.Yu. et al. Determination of anthocyanins and chlorogenic acids in fruits of aronia genus: the ex-perience of chemosystemetics. Khimiya rastitel'nogo syr'ya. 2019; 2: 161–7. doi: 10.14258/jcprm.2019024601 (in Russian)
  4. Cvetanović A., Zengin G., Zeković Z. et al. Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction. Food Chem Toxicol. 2018; 121: 458–66. doi: 10.1016/j.fct.2018.09.045
  5. Brezhneva T.A., Nedoluzhko E.I., Logvinova E.E, Gudkova A.A., Slivkin A.I. The study of biologically active substances in the leaves of black chokeberry. Vestnik VGU. Seriya: Khimiya. Biologiya. Farmatsiya. 2018; 2: 306–11 (in Russian)
  6. Pugacheva O.V., Sviridova O.L., Brezhneva T.A., Slivkin A.I. Validation of a method for the quantification of tannins in black chokeberry leaves. Vestnik VGU. Seriya: Khimiya. Biologiya. Farmatsiya. 2022; 1: 98–104. (in Russian)
  7. Pugacheva, O.V., Brezhneva T.A., Slivkin A.I. Morphological-Anatomic Study Of Aronia Mitchurinii (Aronia Mitchurinii Skvortsov & Maitulina). Sbornik trudov mezhdunarodnoi nauchnoi konferentsii «Ot biokhimii rastenii k biokhimii cheloveka», FGBNU VILAR, Moskva. 2022: 296–300 (in Russian)
  8. State Pharmacopoeia of the Russian Federation. XIV edition. MOSCOW: FEMB, 2018. 7019 с. Available at: http://femb.ru/femb/pharmacopea.php (Accessed 10 May, 2023) (in Russian)
  9. Nikitina A.S., Logvinenko L.A., Nikitina N.V., Nigaryan S.A. Morphometric and histochemical research of melissa officinalis l. Herb from the collection of Nikitsky botanic garden. Farmatsiya i farmakologiya. 2018; 6 (6): 504–34. doi: 10.19163/2307-9266-2018-6-6-504-534 (in Russian)
  10. Lapina A.S., Kurkin V.A., Ryzhov V.M., Tarasenko L.V. The New Aspects In Morphological And Anatomical Diagnostics Of The Herb Monarda Fistulosa L Aspirantskii vestnik Povolzh'ya. 2019; 1–2: 19–26. doi: 10.17816/2072-2354.2019.19.1.19-26 (in Russian)
  11. Zimenikina N.I., Kurkin V.A., Ryzhov V.M., Tarasenko L.V. Luminescent analysis of walnut bark (Juglans regia L.). Sbornik trudov mezhdunarodnoi nauchnoi konferentsii «Ot rasteniya do lekarstvennogo preparata», FGBNU VILAR. 2020: 233–8 (in Russian)
  12. Kovaleva N.A., Trineeva O.V., Gudkova A.A., Slivkin A.I. Study of Morphological Features of Sea Buckthorn Leaves by Luminescent and Stereomicroscopy Methods. Razrabotka i registratsiya lekarstvennykh sredstv. 2022; 11 (1): 123–31. doi: 10.33380/2305-2066-2022-11-1-123-131 (in Russian)
  13. Trineeva O.V., Gudkova A.A., Rudaya M.A. Application of Luminescent Microscopy in Analysis of Anatomic-diagnostic Signs of Fruits of Sea Buckthorn. Razrabotka i registratsiya lekarstvennykh sredstv. 2020; 9 (1): 40–5. doi: 10.33380/2305-2066-2020-9-1-40-45 (in Russian)
  14. Gudkova A.A., Chistyakova A.S., Sinetskaya D.A., Slivkin A.I., Bolgov A.S., Bolgova M.A. Scanning Electron Microscopy in the Analysis of Species of the Genus Persicaria Mill. Razrabotka i registratsiya lekarstvennykh sredstv. 2022; 11 (1): 99–105. doi: 10.33380/2305-2066-2022-11-1-99-105 (in Russian)
  15. Kovaleva N.A., Trineeva O.V. Application of Scanning Electron Microscopy to Study Morphological and Anatomical Features of Sea Buckthorn Leaves. Razrabotka i registratsiya lekarstvennykh sredstv 2023; 12 (2): 79–86. doi: 10.33380/2305-2066-2023-12-2-79-86 (in Russian)

Supplementary files

Supplementary Files
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1. JATS XML
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2. Fig. 1. View of the lower surface of the leaf: а – main vein (×100), б – trichome (×400)

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3. Fig. 2. View of the upper surface of the leaf (×400): а – gland, б – base of gland

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4. Fig. 3. View of the under surface of the leaf lamina: а – leaf surface (×50), б – main vein (×200)

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5. Fig. 4. View of the upper surface of the leaf lamina (×10): а – leaf surface, б – main leaf vein

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6. Fig. 5. Fragments of the leaf aronia Michurina (SEM): а – leaf petiole (×33); б – leaf petiole hairs (×300); в – vein of under surface leaf (×33); г – vein hairs from under surface leaf (×100)

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7. Fig. 6. Fragments of the leaf aronia Michurina (SEM): a, б – lower leaf surface (a – ×33, б – ×100); в – edge of lower leaf side (×300); г – stomata (×1000)

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8. Fig. 7. Fragments of the leaf aronia Michurina (SEM): a – upper leaf surface (×70); б, г – cuticle folding (×300); в – edge of upper leaf surface (×100)

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9. Fig. 8. Fragments of the leaf aronia Michurina (SEM): a – vein of upper leaf surface with glandules (×33); б – unicellular glandules (×200); в – glandular attachment site (×500); г – multicellular gland base (×500)

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