Investigation of the chemical composition of the domestic apple tree fruits by x-ray fluorescence analysis

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Abstract

Introduction. Apple fruits are widely used in the food industry and as non-pharmacopoeial medicinal raw materials. The value of this plant is due to the presence of a large number of biologically active compounds, namely: phenols, flavonoids and tannins. Despite the abundance of information on the chemical composition of this raw material, the study of the elemental composition of apple fruits remains relevant for potential use in the treatment of element deficiency conditions.

Objective: to study the content of chemical elements using X-ray fluorescence analysis in three different parts of apple fruits – in the exocarp, mesocarp and endocarp for four varieties, as well as to identify the nitrate content and measure acidity for these apple varieties.

Material and methods. Apple tree fruits for research were harvested in the Moscow region. After preliminary ashing of the samples, the content of chemical elements was determined by X-ray fluorescence analysis with a silicon drift detector. pH and nitrate ion concentrations were measured using an EXPERT-001 pH meter.

Results. Using X-ray fluorescence analysis (XFA), the elemental composition of four varieties of domestic apple fruits was studied for various parts of the fruit. An increased content of lead was noted in the exocarp of apples of the Spartan variety. A study of the content of nitrate ions and pH was also carried out.

Conclusion. The chemical composition of domestic apple fruits was studied using X-ray fluorescence. The content of chemical elements was different in different parts of the fruit. The lowest content of chemical elements was determined in the mesocarp of the fruit. It is also worth noting the high lead content in the exocarp of Spartan apples.

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About the authors

Kuzmenko Nikolaevich Kuzmenko

Russian State Social University

Email: kuzmenko.mma@mail.ru
ORCID iD: 0000-0001-8214-0055

Doctor of pharmaceutical sciences, professor at the Department of complex of natural science disciplines

Russian Federation, Moscow, st. Wilhelm Pieck, 4 building 1, 129226

Ivan Ivanovich (Jr.) Krasnyuk

Sechenov First Moscow State Medical University (Sechenov University)

Email: krasnyuk.79@mail.ru
ORCID iD: 0000-0001-8557-8829

Doctor of pharmaceutical sciences, professor, Head of the Department of Analytical, Physical and Colloid Chemistry

Russian Federation, Mosсow, Trubetskaya str., 8/2, 119991

Dmitry Olegovich Bokov

Sechenov First Moscow State Medical University (Sechenov University); Federal Research Center of Nutrition and Biotechnology

Email: fmmsu@mail.ru
ORCID iD: 0000-0003-2968-2466

PhD in pharmaceutical sciences, associate professor, associate professor at the Pharmaceutical Natural Sciences Department of Institute of pharmacy named after A.P. Nelyubin, Sechenov First Moscow State Medical University (Sechenov University)

Russian Federation, Mosсow, Trubetskaya str., 8/2, 119991; Moscow, Ustinsky proezd, 2/14, 109240

Olga Nikolaevna Plahotnaya

Sechenov First Moscow State Medical University (Sechenov University)

Email: plahotnaya.o@mail.ru
ORCID iD: 0000-0001-7266-2933

PhD in chemical sciences, associate professor, associate professor at the Department of Analytical, Physical and Colloid Chemistry

Russian Federation, Mosсow, Trubetskaya str., 8/2, 119991

Alexander Alexandrovich Evgrafov

Sechenov First Moscow State Medical University (Sechenov University)

Email: afkx_farm@mail.ru
ORCID iD: 0000-0002-5742-8775

PhD in pharmaceutical sciences, associate professor, associate professor at the Department of Analytical, Physical and Colloid Chemistry 

Russian Federation, Mosсow, Trubetskaya str., 8/2, 119991

Mikhail Nikolaevich Rodin

Sechenov First Moscow State Medical University (Sechenov University)

Email: rodin_m_n@staff.sechenov.ru
ORCID iD: 0000-0001-9532-6879

PhD student, educational master of the Pharmaceutical Natural Sciences Department at Institute

Russian Federation, Mosсow, Trubetskaya str., 8/2, 119991

Ivan Alekseevich Kuzmenko

Scientific Practical Center of Pediatric Psychoneurology

Email: johnny1wises@gmail.com
ORCID iD: 0009-0007-5132-4354

Resident

Russian Federation, Moscow, Energeticheskaya st., 8 building. 2 / 1, 111116

Nadezhda Viktorovna Nesterova

Sechenov First Moscow State Medical University (Sechenov University)

Email: nesterova_n_v_1@staff.sechenov.ru
ORCID iD: 0000-0002-9752-9757

PhD in pharmaceutical sciences, associate professor at the Pharmaceutical Natural Sciences Department

Russian Federation, Mosсow, Trubetskaya str., 8/2, 119991

Irina Alexandrovna Samylina

Sechenov First Moscow State Medical University (Sechenov University)

Author for correspondence.
Email: samylina_i_a@staff.sechenov.ru
ORCID iD: 0000-0002-4895-0203

– Doctor of Pharmaceutical Sciences, corresponding member of the RAS, Professor, professor of the Pharmaceutical Natural Sciences Department 

Russian Federation, Mosсow, Trubetskaya str., 8/2, 119991

References

  1. Chuparina E.V., Gunicheva T.N. State and problems of X-ray fluorescence analysis of plant materials. Analitika i kontrol'. 2004; 8 (3): 211–26 (in Russian).
  2. Chuparina E.V., Aisueva T.S., Zhapova O.I., Antsupova T.P. Determination of metals Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Sr, Ba and Pb in medicinal plants using X-ray fluorescence analysis. Analitika i kontrol'. 2008; 12 (1–2): 2–10 (in Russian).
  3. Grigorev A.V. X-ray fluorescence analysis of plant materials, methods of addition and external standard. Izvestija Rossijskogo gosudarstvennogo pedagogicheskogo universiteta im. A.I. Gercena. 2012; 144: 82–91 (in Russian).
  4. Chuparina E.V., Martynov A.M., Zhapova O.I. X-ray fluorescence analysis of medicinal plants of Eastern Siberia. The Siberian Scientific Medical J. 2008; 3: 98–9 (in Russian).
  5. Shirkin L.A. X-ray fluorescence analysis of environmental objects. Publishing house of the Vladimir State University. 2009; 60 (in Russian).
  6. Samylina I.A., Nesterova N.V. Historical experience and prospects for the use of apple raw materials in medicine and pharmacy. Zdorov'e i obrazovanie v XXI veke. 2015; 17 (4): 251–7 (in Russian).

Supplementary files

Supplementary Files
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1. JATS XML
2. Fig. 1. Calibration curves Note. а – for determining pH, б – for determining the concentration of nitrate ions (concentration of standard solutions: 0,0001, 0,001, 0,01, 0,1 mg/ml).

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3. Fig. 2. Scatter plot to confirm the correctness of the method Note. а – for low concentrations, б – for high concentrations, standard – Elodea canadensis.

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4. Fig. 3. Scatter plot to confirm the correctness of the method Note. а – for low concentrations, б – for high concentrations, standard – grass mixture.

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