Determination of vitamins in seeds and oil of amaranth tailed grown in Uzbekistan

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Abstract

Introduction. Today, a number of scientific studies are being carried out in Uzbekistan on the selection of medicinal plants and their reasonable use, in particular, medicinal species of amaranth cultivated in our country, having a sufficient raw material base, are in the center of attention of scientists.

Material and methods. This research work is devoted to the identification and determination of the amount of vitamins in the seeds and oil of amaranth tailed, grown in the Andijan region of the Republic of Uzbekistan.

Resuts. The research work was carried out using high-performance liquid chromatography-mass spectroscopy (LС-MS), which is considered one of the modern analytical methods, and 12 vitamins were tested, 8 of which were fixed in the contents of the seeds and 4 in the oil. content. High Performance Liquid Chromatography (HPLC) determined the amounts of vitamin E and vitamin D3, which were found to be the most retained among vitamins in oil, and obtained results of pharmaceutical significance.

Conclusion. According to the results of the study, it was concluded that amaranth seeds contain vitamins D3, E, B9, B1, B2, B5, B6, C, of which only D3, E, B9, B6 passed into its oil.

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About the authors

Gulrano Azim kizi Akhmadova

Tashkent Pharmaceutical Institute

Author for correspondence.
Email: gulrano_92@mail.ru
ORCID iD: 0000-0002-1757-1139

Assistant of Department of Pharmaceutical Chemistry, PhD in Pharmaceutical Sciences

Uzbekistan, 100015, Tashkent, ul. Oybek, d. 45

Ismat Kazimovich Azizov

Tashkent Pharmaceutical Institute

Email: gulrano_92@mail.ru
ORCID iD: 0000-0002-1553-4932

Professor of the Department of Pharmaceutical Chemistry of the Tashkent Pharmaceutical Institute, Chairman of the State Center for Expertise and Standardization of Medicinal Products, Research Medical Indication and Medical Technical Agency for the Development of Pharmaceutical Industry at the Ministry of Health and Welfare of the Republic of Uzbekistan for Narcotics Control, Doctor of Pharmaceutical Sciences

Uzbekistan, 100015, Tashkent, ul. Oybek, d. 45

Yodgora Akhmadova

Tashkent Pharmaceutical Institute

Email: y.ahmadova9345@gmail.com
ORCID iD: 0009-0000-9776-1049

Young Researcher-Intern at the Laboratory of Coordination and Biologically Active Compounds

Uzbekistan, 100015, Tashkent, ul. Oybek, d. 45

References

  1. Yokubova N., Hamzayeva N. NUTRITIONAL VEGAN PRODUCTS IN UZBEKISTAN. Galaxy International Interdisciplinary Research J. 2022; 10 (7): 202–4.
  2. Hilolakhan R., Rustam G., Alidjan I. DETERMINATION OF AMINO ACID AND VITAMIN CONTENT OF PHLOMOIDESNUDA AND PHLOMOIDESSPECIOSA (LAMIACEAE) SPECIES. Universum: Chemistry and Biology. 2023; 4 (106): 69–73.
  3. Bozorov S.S. et al. Chemical composition and biological activity of seed oil of amaranth varieties. Nova Biotechnologica et Chimica. 2018; 17 (1): 66–73.
  4. Nodirbek T., Kizi S.S.T. CULTIVATION OF THE MEDICINAL PLANT AMARANTH AND ITS ENTOMOFAUNA. Universum: Chemistry and Biology. 2021; 11–2 (89): 70–3.
  5. Azizov I.K., Akhmadova G.A. Amino acid composition of the seeds of kiwicha (Amaranthus caudatus) growing in Uzbekistan. Farmaciya (Pharmacy). 2021; 70 (7): 37–40.
  6. Ibotov S.K. et al. Lipids of Amaranthus retroflexus and their Biological Activity. Chemistry of Natural Compounds. 2021; 57: 620–6.
  7. Saidkhodzhaeva D. et al. Study of the chemical composition of the amaranth plant by the method of chromotography. E3S Web of Conferences. EDP Sciences. 2023; 390.

Supplementary files

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2. Fig. 1. LC-MS chromatogram of amaranth seed

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3. Fig. 2. LC-MS chromatogram of amaranth oil

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4. Fig. 3. LC-MS chromatogram of amaranth oil (vitamin A, E, D)

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5. Fig. 4. Chromatogram of Vitamin D3 standard

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6. Fig. 5. Chromatogram for determination of vitamin D3 in amaranth oil

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7. Fig. 6. Chromatogram of α-tocopherol working standard sample

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8. Fig. 7. Amaranth oil chromatogram

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