STANDARDIZATION OF THE COMPOSITION OF GROWTH SUBSTRATES FOR OBTAINING AN ANTIFUNGAL ANTIBIOTIC


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Introduction. Natural raw materials with their unstable chemical composition are used as a source of carbohydrates in the biotechnology industry. Synthetic sugars, aryl-substituted xylose derivatives possessing antimicrobial activity do not have this disadvantage. Objective: to investigate synthetic sugars as an additional source of carbohydrates in the composition of a growth medium, as well as a means of protecting the cultivation process of a producer from possible contamination. Material and methods. The investigation object was an antibiotic imbricin producer by the actinomycete Streptomyces imbricatus strain 0112/90, as well as synthetic sugars. Results. It was shown that synthetic sugars could not serve as an additional source of carbohydrate nutrition, but have an antimicrobial effect. Conclusion. The synthetic saccharide (3,4-dinitrophenyl-ß-D-xyloxylopyranoside) at a 0.1% concentration can be recommended for addition to the composition of a growth medium as a means of protection against possible contamination at the fermentation stage.

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作者简介

Elena Yakovleva

Saint Petersburg State Chemopharmaceutical University

Email: elena.yakovleva@pharminnotech.com
Professor of the Department of Biotechnology, Doctor of Biological Sciences, Professor Saint Petersburg, Russian Federation

Vera Kolodyaznaya

Saint Petersburg State Chemopharmaceutical University

Email: vera.kolodyaznaya@pharminnotech.com
Head of the Department of Biotechnology, Candidate of Biological Sciences, Associate Professor Saint Petersburg, Russian Federation

Oksana Topkova

Saint Petersburg State Chemopharmaceutical University

Email: oxana.topkova@pharminnotech.com
Associate Professor of the Department of Biotechnology, Candidate of Biological Sciences, Associate Professor. Saint Petersburg, Russian Federation

参考

  1. Луканин А.В. Инженерная биотехнология: основы технологии микробиологических производств. М.: ИНФРА-М, 2016; 304.
  2. Колодязная В.А., Яковлева Е.П. Технология культивирования продуцентов БАВ. СПб.: изд-во СПХФА, 2016; 72.
  3. Габидова А.Э. Анализ микробиологического риска в производстве пищевых продуктов и лекарственных препаратов. СПб.: Проспект науки, 2016; 384.
  4. Belakhov V., Dor E., Hershenhorn J. et al. Family of Thiomercuric Derivatives of Sugars: Synthesis Fungicidal Herbicidal Activity and Application to the X-Ray Structure Determination of the Corresponding Enzymes. Israel Journal of Chemistry, 2000; 40 (3-4); 177-88.
  5. Тихомирова О.М., Гурина С.В. Промышленная асептика в биотехнологическом производстве. СПб.: изд-во СПХФА, 2014; 80.
  6. Фрешни Р.Я. Культура животных клеток: практическое руководство. М.: БИНОМ. Лаборатория знаний, 2011; 691.
  7. Бакулин М.К., Грудцына А.С., Плетнева А.Ю. и др. Характеристика антибиотической продуктивности бактерий рода Streptomyces при культивировании в среде с добавлением карбогала и перфторметилдекалина. Биотехнология, 2006; 5; 39-44.
  8. Buschke N., Schafer R., Becker J., Witmann C. Metabolic engineering of industrial platform microorganisms for biorefinery applications - optimization of substrate spectrum and process biosintesis by rational and evolutive strategies. Bioresour. Technol., 2013; 135; 544-4.
  9. Mechaly A., Belachov V., Shoham Y., Baasov T. An efficient chemical-enzymatic synthesis of 4-nitrophenyl β-xylobioside: a chromogenic substrate for xylanases. Carbohydrate Research, 1997 (304); 111-5.

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