DETERMINATION OF USNIC ACID IN THE BLASTEMAS OF A LICHEN OF THE GENUS CLADONIA BY HPLC/UV


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Introduction. Cup moss that is a combination of lichens of the genus Cladonia contains biologically active substances (usnic acids, phenolic compounds, etc.) that have antibacterial and antiviral properties. The sodium salts of usnic acid have long been used in pharmaceutical practice. Various methods (TLC, ELISA, HPLC/MS, etc.) are used to determine usnic acid in plant materials. Cup moss is not an officinal raw material and its estimation by the content of usnic acid is absent. Objective: to develop and validate a procedure for the quantitative determination of usnic acid in the blastemas of the lichen of the genus Cladonia. Material and methods. The investigation object was cup moss samples collected in the Republic of Sakha (Yakutia). Usnic acid was determined by HPLC-UV. Results. A procedure for the quantitative determination of usnic acid in the blastemas of the lichen of the genus Cladonia was developed and validated by HPLC with a measurement error of ±4.47%. Conclusion. The developed method was shown to be promising in analyzing the plant substance for the levels of usnic acid. With its usage, it was found that the content of usnic acid in the blastemas of the cup moss growing in different regions of Yakutia ranges from 0.88 to 1.02%.

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作者简介

Alexander Nikulin

Collective Usage Center (Research Educational Center), People's Friendship University of Russia

Email: r251@yandex.ru
Head of the Laboratory of physicochemical method; PhD (Chem.). 6, Miklukho-Maklai St., Moscow 117198, Russian Federation

Sargylana Yamshchikova

Collective Usage Center (Research Educational Center), People's Friendship University of Russia; M.K. Ammosov North-Eastern Federal University

Email: ssontoeva@gmail.ru
postgraduate student; assistant of department of pharmacology and pharmacy of Medical institute 6, Miklukho-Maklai St., Moscow 117198, Russian Federation; 27, Oiunsky St., Moscow 677000, Russian Federation

Olga Potanina

Collective Usage Center (Research Educational Center), People's Friendship University of Russia

Email: microly@mail.ru
Head of the pharmaceutical chemistry and pharmacognosy chair, Director of the Research and Development department, Doctor of Pharmaceutical Sciences. 6, Miklukho-Maklai St., Moscow 117198, Russian Federation

Rimma Abramovich

Collective Usage Center (Research Educational Center), People's Friendship University of Russia

Email: abr-rimma@yandex.ru
Head of drugs technology and pharmacy management chair of Advanced Training Faculty of medical employee, Director, Doctor of Pharmaceutical Sciences 6, Miklukho-Maklai St., Moscow 117198, Russian Federation

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