DETERMINATION OF USNIC ACID IN THE BLASTEMAS OF A LICHEN OF THE GENUS CLADONIA BY HPLC/UV


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Introduction. Cup moss that is a combination of lichens of the genus Cladonia contains biologically active substances (usnic acids, phenolic compounds, etc.) that have antibacterial and antiviral properties. The sodium salts of usnic acid have long been used in pharmaceutical practice. Various methods (TLC, ELISA, HPLC/MS, etc.) are used to determine usnic acid in plant materials. Cup moss is not an officinal raw material and its estimation by the content of usnic acid is absent. Objective: to develop and validate a procedure for the quantitative determination of usnic acid in the blastemas of the lichen of the genus Cladonia. Material and methods. The investigation object was cup moss samples collected in the Republic of Sakha (Yakutia). Usnic acid was determined by HPLC-UV. Results. A procedure for the quantitative determination of usnic acid in the blastemas of the lichen of the genus Cladonia was developed and validated by HPLC with a measurement error of ±4.47%. Conclusion. The developed method was shown to be promising in analyzing the plant substance for the levels of usnic acid. With its usage, it was found that the content of usnic acid in the blastemas of the cup moss growing in different regions of Yakutia ranges from 0.88 to 1.02%.

Full Text

Restricted Access

About the authors

Alexander Vladimirovich Nikulin

Collective Usage Center (Research Educational Center), People's Friendship University of Russia

Email: r251@yandex.ru
Head of the Laboratory of physicochemical method; PhD (Chem.). 6, Miklukho-Maklai St., Moscow 117198, Russian Federation

Sargylana Ivanovna Yamshchikova

Collective Usage Center (Research Educational Center), People's Friendship University of Russia; M.K. Ammosov North-Eastern Federal University

Email: ssontoeva@gmail.ru
postgraduate student; assistant of department of pharmacology and pharmacy of Medical institute 6, Miklukho-Maklai St., Moscow 117198, Russian Federation; 27, Oiunsky St., Moscow 677000, Russian Federation

Olga Georgievna Potanina

Collective Usage Center (Research Educational Center), People's Friendship University of Russia

Email: microly@mail.ru
Head of the pharmaceutical chemistry and pharmacognosy chair, Director of the Research and Development department, Doctor of Pharmaceutical Sciences. 6, Miklukho-Maklai St., Moscow 117198, Russian Federation

Rimma Aleksandrovna Abramovich

Collective Usage Center (Research Educational Center), People's Friendship University of Russia

Email: abr-rimma@yandex.ru
Head of drugs technology and pharmacy management chair of Advanced Training Faculty of medical employee, Director, Doctor of Pharmaceutical Sciences 6, Miklukho-Maklai St., Moscow 117198, Russian Federation

References

  1. Подтероб А.П. Химический состав лишайников и их медицинское применение. Химико-фармацевтический журнал. 2008; 42 (10): 32-8. https://doi.org/10.30906/0023-1134-2008-42-10-32-38
  2. Дембицкий В.М., Толстиков Г.А. Органические метаболиты лишайников. Новосибирск: Издательство СО РАН, Филиал «Гео»; 2005.
  3. Павлова Е.С., Павлов Н.Г. Лишайники рода цетрарии (Cetraria) и кладонии (Cladonia) в экспериментальном исследовании. Материалы III Международной научной интернет-конференции: в 2 томах. Биотехнология. Взгляд в будущее, Казань, 2014: 36-42.
  4. Загоскина Н.В., НиколаеваТ.Н., Лапшин П.В., Заварзин А.А., Заварзина А.Г. Водорастворимые фенольные соединения у лишайников. Микробиология. 2013; 82 (4): 434-41. https:// doi.org/10.7868/S0026365613030166
  5. Чахирова В.А., Верещагина В.В., Погорелов В.И. Определение содержания биологически активных веществ в сырье ягеля (оленьего мха). Известия вузов. СевероКавказский регион. Естественные науки. Спецвыпуск Фармакология. 2006; 58-9.
  6. Chikita F. Culberson, Hor-Dur Kristinsson. A standardized method for the identification of lichen products. Journal of chromatography, 1970; 46: 85-93.
  7. Буркин А.А., Кононенко Г.П., Толпышева Т.Ю. Иммуноферментный анализ усниновой кислоты в лишайниках. Прикладная биохимия и микробиология, 2013; 49 (3): 322-8. https://doi.org/10.7868/S0555109913030069
  8. Прокопьев И.А., Шаварда И.Л., Филиппова Г.В., Шеин А.А. Применение высокоэффективной жидкостной хроматографии для определения содержания вторичных метаболитов лишайников. Журнал аналитической химии, 2017; 72 (11): 1025-31. https://doi.org/10.7868/ S004445021711007X
  9. Feige G.B., Lumbsch H.T. Identification of lichen substances by a standardized high-performance liquid chromatographic method. Journal of Chromatography, 1993; 646: 417-27.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2020 Russkiy Vrach Publishing House

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies