Architecture of promoters of house-keeping genes in polytene chromosome interbands of drosophila melanogaster

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This is the first study to investigate the molecular-genetic organization of polytene chromosome interbands located on both molecular and cytological maps of Drosophila genome. The majority of the studied interbands contained one gene with a single transcription initiation site; the remaining interbands contained one gene with several alternative promoters, two or more unidirectional genes, and “head-to-head” arranged genes. In addition, intricately arranged interbands containing three or more genes in both unidirectional and bidirectional orientation were found. Insulator proteins, ORC, P-insertions, DNase I hypersensitive sites, and other open chromatin structures were situated in the promoter region of the genes located in the interbands. This area is critical for the formation of the interband, an open chromatin region in which gene transcription and replication are combined.

About the authors

T. Yu. Zykova

Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences

Author for correspondence.

Russian Federation, acad. Lavrentiev Ave. 8/2, Novosibirsk, 630090

V. G. Levitsky

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University


Russian Federation, 10, Lavrentjev avenue, Novosibirsk, 630090; 1, Pirogova street, Novosibirsk, 630090

I. F. Zhimulev

Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University


Russian Federation, acad. Lavrentiev Ave. 8/2, Novosibirsk, 630090; 1, Pirogova street, Novosibirsk, 630090

Academician of the RAS


  1. Kharchenko P. V., Alekseyenko A. A., Schwartz Y. B., et al. Comprehensive Analysis of the Chromatin Landscape in Drosophila melanogaster // Nature. 2011. V. 471. № 7339. P. 480-485.
  2. Zhimulev I. F., Zykova T. Y., Goncharov F. P., Khoro- shko V. A., Demakova O. V., Semeshin V. F., Pok- holkova G. V., Boldyreva L. V., Demidova D. S., Babenko V. N., Demakov S. A., Belyaeva E. S. Genetic Organization of Interphase Chromosome Bands and Interbands in Drosophila melanogaster // PLoS One. 2014. V. 9. № 7. e101631.
  3. Zhimulev I., Belyaeva E., Makunin I., Pirrotta V., Volkova E., Alekseyenko A. Influence of the SuUR Gene on Intercalary Heterochromatin in Drosophila mela- nogaster Polytene Chromosomes // Chromosoma. 2003. V. 111. № 6. P 377-398.
  4. Vatolina T.Yu., Boldyreva L. V., Demakova O. V., Demakov S. A., Kokoza E. B., Semeshin V. F., Babenko V. N., Goncharov F. P., Belyaeva E. S., Zhimu- lev I. F. Identical Functional Organization of Cell Line Interphase and Polytene Chromosomes in Drosophila melanogaster // PLoS One. 2011. V. 6. № 10. e25960.
  5. Khoroshko V. A., Levitsky V. G., Zykova T. Y., Antonenko O. V., Belyaeva E. S., Zhimulev I. F. Chromatin Heterogeneity and Distribution of Regulatory Elements in the Late-Replicating Intercalary Heterochromatin Domains of Drosophila melanogaster Chromosomes // PLoS One. 2016. V. 11. № 6. e0157147.
  6. Zhimulev I. F., Belyaeva E. S., Semeshin V. F., Koryakov D. E., Demakov S. A., Demakova O. V., Pokholkova G. V., Andreyeva E. N. Polytene Chromosomes: 70 Years of Genetic Research // Int. Rev. Cytol. 2004. V. 241. P. 203-275.
  7. Beermann W. Chromomeres and Genes // Results and Problems in Cell Differentiation. 1972. V. 4. P. 1-33.
  8. Зыкова Т., Попова О., Хорошко В., Левицкий В., Лавров С., Жимулев И. Генетическая организация доменов открытого типа, расположенных в междисках политенных хромосом дрозофилы // ДАН. 2018. Т. 483. № 1. С. 98-102.
  9. Zykova T., Levitsky V., Belyaeva E., Zhimulev I. Poly- tene Chromosomes – A Portrait of Functional Organization of the Drosophila Genome // Curr. Genomics. 2018. V. 19. № 3. P 179-191.
  10. Pokholkova G., Demakov S., Andreenkov O., Andreen- kova N., Volkova E., Belyaeva E., Zhimulev I. Tethering of CHROMATOR and dCTCF Proteins Results in Decompaction of Condensed Bands in the Drosophila melanogaster Polytene Chromosomes but Does Not Affect Their Transcription and Replication Timing // PLoS One. 2018. V. 13. № 4. e0192634.
  11. Vogelmann J., Le Gall A., Dejardin S., Allemand F., Gamot A., Labesse G., Cuvier O., Negre N., Cohen- Gonsaud M., Margeat E., Ndllmann M. Chromatin Insulator Factors Involved in Long-Range DNA Interactions and Their Role in the Folding of the Drosophila Genome // PLoS Genet. 2014. V. 10. № 8. e1004544.
  12. Henriques T., Gilchrist D., Nechaev S., Bern M., Muse G., Burkholder A., Fargo D., Adelman K. Stable Pausing by RNA Polymerase II Provides an Opportunity to Target and Integrate Regulatory Signals // Mol. Cell. 2013. V. 52. № 4. P 517-528.
  13. Nechaev S., Fargo D., dos Santos G., Liu L., Gao Y., Adelman K. Global Analysis of Short RNAs Reveals Widespread Promoter-Proximal Stalling and Arrest of Pol II in Drosophila // Science. 2010. V. 327. № 5963. P. 335-338.
  14. Eaton M. L., Prinz J. A., MacAlpine H.K., Tretyakov G., Kharchenko P. V., MacAlpine D. M. Chromatin Signatures of the Drosophila Replication Program // Genome Res. 2011. V. 21. № 2. P. 164-174.
  15. Hoskins R. A., Landolin J. M., Brown J. B., Sandler J. E., Takahashi H., et al. Genome-Wide Analysis of Promoter Architecture in Drosophila melanogaster // Genome Res. 2011. V. 21. № 2. P. 182-192



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