Study оf dCTCF insulator activity in Drosophila melanogaster model systems

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Abstract


Using Drosophila transgenic model systems, we have shown that the four binding sites for the architectural protein dCTCF themselves have no insulator activity. They cannot block enhancers and protect from Polycomb-dependent repression. The results suggest that in the known Drosophila insulators, the dCTCF protein functions in cooperation with other architectural proteins.


About the authors

N. E. Postika

Institute of Gene Biology of the Russian Academy of Sciences

Author for correspondence.
Email: nk.postika@gmail.com

Russian Federation, 34/5, Vavilova street, Moscow, 119334

T. A. Ivlieva

Institute of Gene Biology of the Russian Academy of Sciences

Email: nk.postika@gmail.com

Russian Federation, 34/5, Vavilova street, Moscow, 119334

P. G. Georgiev

Institute of Gene Biology of the Russian Academy of Sciences

Email: nk.postika@gmail.com

Russian Federation, 34/5, Vavilova street, Moscow, 119334

Academician of the Russian Academy of Sciences

O. V. Kyrchanova

Institute of Gene Biology of the Russian Academy of Sciences

Email: nk.postika@gmail.com

Russian Federation, 34/5, Vavilova street, Moscow, 119334

References

  1. Ali T., Renkawitz R., Bartkuhn M. // Curr. Opin. Genet. Dev. 2016. V. 37. P. 17-26. doi: 10.1016/j.gde. 2015.11.009.
  2. Moon H., Filippova G., Loukinov D., Pugacheva E., Chen Q., Smith S. T., Munhall A., Grewe B., Bartkuhn M., Arnold R., Burke L. J., Renkawitz-Pohl R., Ohlsson R., Zhou J., Renkawitz R., Lobanenkov V. // EMBO Rept. 2005. V. 6. № 2. Р. 165-170.
  3. Kyrchanova O., Toshchakov S., Podstreshnaya Y., Parshikov A., Georgiev P. // Mol. Cell. Biol. 2008. V. 28. № 12. P. 4188-4195. doi: 10.1128/MCB.00229-08.
  4. Bonchuk A., Maksimenko O., Kyrchanova O., Ivlieva T., Mogila V., Deshpande G., Wolle D., Schedl P., Georgiev P. // BMC Biol. 2015. V. 13. P. 63. doi: 10.1186/s12915-015-0168-7.
  5. Holohan E.E., Kwong C., Adryan B., et al. // PLoS Genet. 2007. V. 3. № 7. e112.
  6. Maksimenko O., Kyrchanova O., Bonchuk A., Stakhov V., Parshikov A., Georgiev P. // Epigenetics. 2014. V. 9. № 9. P. 1261-1270. doi: 10.4161/epi.32086.
  7. Kyrchanova O., Georgiev P. // FEBS Lett. 2014. V. 588. № 1. P. 8-14. doi: 10.1016/j.febslet.2013.10.039.
  8. Karess R.E., Rubin G. M. // Cell. 1984. V. 38. № 1. P. 135-146.
  9. Kostyuchenko M., Savitskaya E., Koryagina E., Melnikova L., Karakozova M., Georgiev P. // Chromosoma. 2009. V. 118. № 5. P. 665-674.
  10. Kyrchanova O., Maksimenko O., Stakhov V., Ivlieva T., Parshikov A., Studitsky V. M., Georgiev P. // PLoS Genet. 2013. V. 9. № 7. e1003606. doi: 10.1007/s00412-009-0226-4.
  11. Gruzdeva N., Kyrchanova O., Parshikov A., Kullyev A., Georgiev P. // Mol. Cell. Biol. 2005. V. 25. № 9. P. 3682-3689.
  12. Kyrchanova O., Mogila V., Wolle D., Deshpande G., Parshikov A., Cléard F., Karch F., Schedl.P, Georgiev P. // PLoS Genet. 2016. V. 12. № 7. e1006188. doi: 10.1371/journal.pgen.1006188.
  13. Федотова А.А., Бончук А.Н., Могила В.A., Георгиев П.Г. // Acta Naturae. 2017. Т. 9. № 2. С. 50-61.
  14. Kyrchanova O., Zolotarev N., Mogila V., Maksimenko O., Schedl P., Georgiev P. // Development. 2017. V. 144. № 14. P. 2663-2672. doi: 10.1242/dev.149815.
  15. Erokhin M., Davydova A., Kyrchanova O., Parshikov A., Georgiev P., Chetverina D. // Development. 2011. V. 138. № 18. P. 4097-4106. doi: 10.1242/dev.062836.

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