Physiological characteristics suspension culture of Rhodiola rosea L. at cultivation on the modified nutrient medium

To use cell plant cultures in the production of biologically active substances, one must know their properties, including the patterns of growth and biosynthetic activity. As a result of the study of the physiological features of the Rhodiola rosea L. culture of the Rr(S) 2013VILAR strain in the dynamics of biomass accumulation under conditions of deep cultivation on a macrosolated nutrient medium, the duration of culture growth was determined to be 15-20 days. The process of culture growth under conditions of deep cultivation can be described by a growth curve having an S-shape. The factor of physiological heterogeneity of the suspension culture is noted, which can significantly influence the final results on biomass accumulation and its bioproductivity. It was found that the maximum consumption of oxygen by the cell culture is 12-10-4 mol/l-h and falls on the 8th day of growth, when the cells are in the phase of regular growth and begin to actively share. To maintain the active growth of the suspension culture of Rhodiola rosea L. strain Rr(S) 2013 VILAR in the cultivation vessels, it is necessary to create a mass transfer coefficient of 8 h-1. It is shown that the accumulation of the sum of phenylpropanoids in the cell culture of Rhodiola rosea L. of the Rr(S) 2013VILAR strain occurs in parallel with the growth of biomass.

Rhodlola rosea L. (R. rosea), cell culture of Rhodiola rosea (R. rosea), suspension culture, nutrient medium, respiratory activity, phenylpropanoids