The study of the composition of the fatty oil and the content of microelements in Oenothera biennis L.


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Oenothera biennis (biennial evening primrose) - Oenothera biennis L. is a biennial herbaceous plant is one of willowherb family (Onagraceae). Medicinal raw materials are seeds that are harvested during the ripening of the fruit in the second year of vegetation. The object of the study was the fruits of the samples of the collection of two-year oslinnik harvested in 2013-15 on the experimental site of VILAR. The studies were performed in 3 repetitions. Their average data and standard deviations were calculated. The study of the component composition of O. biennis L. fatty oil was carried out by gas-liquid chromatography after the preliminary transfer of fatty acids into methyl esters according to GOST 30418-96. To study the elemental chemical composition of Oenothera biennis L., the samples of seeds recommended as medicinal raw materials were studied. Method of analysis: inductively coupled plasma mass spectrometry (Nexion 300 D quadrupole mass spectrometer (Perkin Elmer, USA). Fatty acid composition of seeds of promising samples of O. biennis L., cultivated in VILAR, characterized by a high content of linoleic (70,0 to 73,9% in oil) and Y-linolenic acid (8,12-9,25% in oil). The analysis of mineral composition showed the presence in the fruit, evening primrose macroelements: Ca, K, Mg, Fe, Mn, and P. Among micronutrients, there are high values of Zn, Mn, Sr, Fe, Al, Si. The content of the toxic element of lead in the studied samples of evening primrose does not exceed the permissible concentration for tea and medicinal plant materials, which indicates its environmental safety. A study of fatty acid composition of seeds of Oenothera biennis L. allowed to identify the most promising samples, characterized by high productivity and yield fatty oil. These requirements are met by sample number 3-89: seed yield 1,9 t/ha; oil content in seeds - 23,7%; high content of linoleic (73,9% in oil) and Y-linolenic acid (9,25% in oil). Analysis of the mineral composition of O. biennis L. showed the presence of 25 mineral elements, among which both essential and toxic elements were found.

Full Text

Restricted Access

About the authors

S. A Totskaya

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Ph.D. (Biol.), Senior Research Scientist, Agrobiotechnology Department

M. Yu Grjaznov

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Ph.D. (Biol.), Senior Research Scientist, Agrobiotechnology Department

S. I Cyganok

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Dr.Sc. (Biol.), Chief Research Scientist, Agrobiotechnology Department

O. M Savchenko

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Email: swamprat@rambler.ru
Ph.D. (Agricult.), Senior Research Scientist, Agrobiotechnology Department

References

  1. Флора СССР. Т.15 / Под ред. А.А. Федорова. М.-Л.: Наука. 1954. С. 628-629.
  2. Климахин Г.И., Тоцкая С.А., Фонин В.С., Семикин В.В., Хазиева Ф.М. Биоразнообразие Oenothera biennis L. Состояние и перспективы интродукции ослинника двулетнего (Oenothera biennis L.) // Вопросы биологической медицинской и фармацевтической химии. 2012. № 7. С. 44-46.
  3. Вандышев В.В., Грицюк Я.А., Климахин Г.И. К фармокологическому изучению энотеры двулетней // Актуальные проблемы создания новых лекарственных препаратов природного происхождения: тезисы докладов 4го Междунар. съезда. СПб. 2002. С. 202-205.
  4. Глушенкова А.И., Уличенко Н.Т. Нейтральные липиды Oenothera biennis L. // Химия природных соединений, 1999. № 3. С. 317-321.
  5. Климахин Г.И., Семикин В.В., Фонин В.С. и др. Динамика образования жирного масла в семенах ослинника двулетнего (Oenothera biennis L.) в условиях культуры // Лекарственное растениеводство: сб. научных трудов. М. 2006. С. 202-206.
  6. Helling A. Composition fopr supporting the treatment of atopic dermatitis // Appl. 1997. S. 19, 715, 878.
  7. Scott C.A., Horrobin D.F. Fatty acis for prevention of side effects of chemotherapy // PCT Int. Appl. 1998. S. 621.
  8. Писарев Д.И., Жилякова Е.Т., Нетребенко Н.Н., Тохтарь В.К., Новиков О.О., Сорокопудов В.Н. Жирнокислотный состав семян Oenothera biennis L. Флоры Белгородской области. // Химия растительного сырья. 2010. № 1. С. 195-196.
  9. Shi Y., Luo K., He J. Comparison and analysis on nutritive element in wild and planted Oenothera biennis L. // Guangdong weilinf Yuansu Kexue. 1998. S. 59-61.
  10. Deng Yu Cheng, Hua Hui Ming, Li Jun. Lapinskas // Economic Botany. 2001. V. 55(1). P. 83-91.
  11. Awaisheh S.S., Khalifeh M.S., Al-Ruwaili M.A., Khalil O.M., Al-Ameri O.H., Al-Groom R. Effect of supplementation of probiotics and phytosterols alone or in combination on serum and hepatic lipid profiles and thyroid hormones of hypercholesterolemic rats // Journal of dairy science. 2013. V. 96. № 1. P. 9-15.
  12. Захарова И.Н., Суркова Е.Н. Роль полиненасыщенных жирных кислот в формировании здоровья у детей // Педиатрия. 2009. № 88(6). С. 84-91.
  13. Терешина Е.В. Роль жирных кислот в развитии возрастного окислительного стресса // Гипотеза. Успехи герантологии. 2007. № 20(1). С. 59-65.
  14. Пилат Т.Л., Иванов АА. Биологически активные добавки к пище (теория, производство, применение). М. 2002. 710 с.
  15. Шугалей И.В., Гарабаджиу А.В., Илюшин М.А., Судариков А.М. Некоторые аспекты влияния алюминия и его соединений на живые организмы // Экологическая химия. 2012. № 21(3). С. 172-186.
  16. Санитарные правила и нормы СанПин 2.3.2/1078-01. «Гигиенические требования безопасности и пищевой ценности пищевых продуктов от 06.11.2001 г. с изменениями от 31.05.2002 г.
  17. Государственная фармакопея Российской Федерации. XIV изд. ОФС 15.3.0009.15. М. 2018.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2019 Russkiy Vrach Publishing House