Antioxidant activity of the dry extract from Silene jeniseensis Willd at experimental immunodeficiency


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Abstract

Rationale. Many vital metabolic and physiological processes occurring in the body are closely related to free radical oxidation. Toxic peroxide free radicals and reactive oxygen species damage important structural and functional proteins and lipids, enzyme and membrane systems of cells that results in the suppression of the functional activity of immunocompetent cells and emergence of various immunopathological processes. The use of new immunomodulators based on plant raw materials with antioxidant activity is relevant for their correction. The aim of this work is to evaluate the antioxidant properties of the dry Silene jeniseensis Willd extract in experimental immunodeficiency. Material and methods. Studies were conducted on Fl(CBAxC57BI/6) mice. Immunodeficiency was simulated by intraperitoneal administration of cyclophosphamide. S. jeniseensis extract was administered at a dose of 100 mg/kg in association with cyclophosphamide. The intensity of lipid peroxidation and the state of the endogenous antioxidant system were evaluated by determining of the malonic dialdehyde (MDA) and reduced glutathione (GSH) content and catalase activity in the spleen homogenate. The membrane-stabilizing activity was evaluated using a model of peroxide hemolysis with a suspension of red blood cells from donor blood. Antiradical activity was determined by the ability of the remedy to bind 2,2'-diphenyl-l-picrylhydrazyl (DPPH'), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS,+), superoxide anion radical (О2”) and Fe2+. Results. The introduction of S. jeniseensis extract reduced the severity of oxidative stress in immunosuppression: the MDA content decreased by 2.0 times; the catalase activity and GSH content increased by 2.3 and 1.5 times, respectively, compared to the level of suppression. S. jeniseensis is characterized by pronounced membrane-stabilizing activity, shows the anti-radical effect against DPPH1, ABTS’+ and O2” radicals, also it has Fe2+- chelating activity. Conclusions. S. jeniseensis extract has a pronounced antioxidant activity in experimental immunodeficiency which is manifested by the decrease of the malonic dialdehyde concentration, increase of catalase activity and increase of the reduced glutathione content, also it has the membrane-stabilizing and anti-radical effects.

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About the authors

A. V Khalzanova

Buryat State University named after Dorzhi Banzarov

Email: halzanova-79@mail.ru
Post-graduate Student, Department of Pharmacology and Traditional Medicine, Medical Institute Ulan-Ude

A. A Toropova

Institute of General and Experimental Biology SB RAS; Buryat State University named after Dorzhi Banzarov

Email: anyuta-tor@mail.ru
Ph.D. (Biol.), Research Scientist, Laboratory of the Biologically Active Substances Safety, Institute of General and Experimental Biology SB RAS; Senior Lecturer, Department of Infectious Diseases, Medical institute, Buryat State University named after Dorzhi Banzarov Ulan-Ude

V. B Khobrakova

Institute of General and Experimental Biology SB RAS; Buryat State University named after Dorzhi Banzarov

Email: val0808@mail.ru
Dr.Sc. (Biol.), Associate Professor, Head of the Laboratory of Experimental Pharmacology, Institute of General and Experimental Biology SB RAS; Professor, Department of Human General Pathology, Medical Institute, Buryat State University named after Dorzhi Banzarov Ulan-Ude

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