AEROGELS' BIOSAFETY ASSESSMENT IN LOCAL TREATMENT OF PURULENT WOUNDS

The work is devoted to the study of cerium-based experimental antiseptic samples of draining sorbents in the form of aerogels obtained by lyophilic drying. Their antimicrobial and sorption properties in vitro conditions were studied, and biosafety in purulent wounds' local treatment in the inflammatory phase in vivo conditions were assessed. The studies were carried out in comparative aspect with the powdered sorbent "Klarisorb-A" used in clinical practice, intended for treatment of various etiologies exudating and bleeding wounds, in particular, infected wounds in the first phase of the wound process with microbial contamination level of 104 - 106 CFU/cm2. It was found that there were no fundamental differences in restoration of dermis layers and scar tissue'sformationwith the local application of various draining sorbents, and the use of cerium leads to increase of mast cells' number in dermis, which indicates a higher local immunity. The technique of purulent woundforming during in vivo experiment was developed for conducting research on elaboration of new types of powdered wound coatings. The use of fixing rings in purulent wound's formation in comparison with the experiment without their application, showed that dermis layers' cross-section for sewing rings complicatesplanimetry during the experiment, but also creates conditions for subcutaneous pocket'sformation. These experimental features do not stop the epithelialization process at the cellular level, but significantly slow down overall healing process and stimulate more massive scar tissue'sformation.

cerium (III) chloride heptahydrate, alginates, purulent wound'sforming, lyophilic drying, bio-coating, aerogel, draining sorbent