Study of the stability of 2,6-di- tert -butyl-4-methylhydroxybenzene in biological material

A. P. Chernova; Чернова А. П.; M. K. Elizarova; Елизарова М. К.; V. K. Shormanov; Шорманов В. К.; O. I. Pugacheva; Пугачёва О. И.

doi:10.29296/25877313-2022-01-03

Study of the stability of 2,6-di-tert-butyl-4-methylhydroxybenzene in biological material

Authors: Chernova A.P.¹, Elizarova M.K.², Shormanov V.K.³, Pugacheva O.I.³
Affiliations:
1. National Research Tomsk Polytechnic University
2. Yeisk Medical College of the Ministry of Health of the Krasnodar Territory
3. Kursk State Medical University
Issue: Vol 25, No 1 (2022)
Pages: 21-27
Section: Articles
URL: https://journals.eco-vector.com/1560-9596/article/view/112923
DOI: https://doi.org/10.29296/25877313-2022-01-03
ID: 112923

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Abstract

Aim purpose. Study of stability of 2,6-di-ferf-butyl-4-methylhydroxybenzene in biological material. Methods. The studies were carried out by GC-MS, TLC and UV spectrophotometry.2,6-di-ferf-butyl-4-methylhydroxybenzene was isolated from the biomatrix (liver tissue) by infusion with a mixture of ethyl acetate-acetone (7:3). The analyte was purified by combining extraction (water-ethyl acetate system) and semi-preparative column chromatography (sorbent - silica gel L 40/100 pm, eluent -hexane-acetone (9.5:0.5)), Results. It was found that at -22oC, 2oC, 10oC, 20oC and 30oC. 2,6-di-ferf-butyl-4-methylhydroxybenzene remains in the biomaterial for 469, 371, 315, 301 and 259 days, respectively. The possibility of mathematical description of the dynamics of analyte decomposition in biomaterial at the indicated temperatures using the hyperbole equation has been studied. Coefficients in the equation of hyperbola (km), Calculated from the results of the experiment, for temperatures of -22oC, 2oC, 10oC, 20oC and 30oC were respectively 11415, 7508, 4983, 3696 and 2156. A linear dependence of km was found. from the storage temperature tü, which is described by the equation: km = 182,24(50-f°) -1702,36. Conclusion. The dynamics of decomposition of 2,6-di-ferf-butyl-4-methylhydroxybenzene in the liver tissue at -22oC - + 30oC, which can be described by the hyperbole equation, is shown. The possibility of predicting the nature of analyte stability in biomaterial in the specified temperature range is shown.

Keywords

2,6-di-tert-butyl-4-methylhydroxybenzene, biological material, storability, dynamics of decomposition, prediction of stability

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About the authors

A. P. Chernova

National Research Tomsk Polytechnic University

Email: apa2004@mail.ru

Ph.D. (Chem.), Associate Professor

Russian Federation,

M. K. Elizarova

Yeisk Medical College of the Ministry of Health of the Krasnodar Territory

Email: goukkemk@mail.ru

Ph.D. (Pharm.)

Russian Federation,

V. K. Shormanov

Kursk State Medical University

Email: r-wladimir@yandex.ru

Dr.Sc. (Pharm.), Professor

Russian Federation,

O. I. Pugacheva

Kursk State Medical University

Author for correspondence.
Email: lnpugacheva@yandex.ru

Post-graduate Student

Russian Federation,

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