Standardization of anti-inflammatory herb tea

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The composition of anti-inflammatory herbal tea intended for the treatment and prevention of chronic colitis includes Bergenia crassifolia (L.) Fritsch. black leaves, Matricaria chamomilla L. flowers, Achillea millefolium L. herb and Mentha piperita L. leaves. Standardization of anti-inflammatory herbal tea is the purpose of this work. Material and methods. Samples of herbal tea produced by SMEs "Arura" (Ulan-Ude) were used. The microcolumn HPLC method was used to investigate the composition of phenolics. The total contents of biologically active substances were determined according to pharmacopoeial methods. Results. The contents of arbutin, gallic acid, bergenin, chlorogenic acid, rutin, rosmarinic acid in herbal tea were 1.10, 0.12, 0.34, 0.05, 0.46 %, respectively. The total contents of flavonoids, phenolic acids, tannins, triterpenes, anthocyanins, organic acids, water-soluble polysaccharides, pectin substances, amino acids in herbal tea were 2.96, 0.21, 9.45, 1.24, 0.21, 3.90, 4.10, 0.90, 0.14%, respectively. A differential method for the quantitative determination of flavonoids was adapted to standardize the herbal tea, the error of a single determination of the content of flavonoids did not exceed ±4.45%. Diagnostically significant microscopic features of herbal tea were established. Minimal content levels of the main groups of biologically active substances in anti-inflammatory herbal tea have been established. Thus, total contents of flavonoids (luteolin equivalents), tannins and arbutin were not less than 2.0, 8.0, 0.5%, respectively. Conclusions. Anti-inflammatory herbal tea is proposed to standardize according to the sum of phenolics: flavonoids (luteolin equivalents), tannins and arbutin. Minimal content levels of the main phenolics, diagnostically significant microscopic features of herbal tea were established.

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About the authors

P. B. Lubsundorzhieva

Institute of General and Experimental Biology of the SB RAS

Author for correspondence.

Dr.Sc. (Pharm.), Laboratory of Biomedical Research

Ulan-Ude, Russia

N. I. Kashenko

Institute of General and Experimental Biology of the SB RAS


Dr.Sc. (Pharm.), Laboratory of Biomedical Research

Ulan-Ude, Russia

T. D. Dargaeva

All-Russian Scientific Research of Medicinal and Aromatic Plants


Dr.Sc. (Pharm.), Professor, Department of Standardization and Certification

Moscow, Russia


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Supplementary files

Supplementary Files
1. Fig. 1. Microscopy of collection particles passing through a sieve with a hole diameter of 2 mm (notations in the text). Magnification of the light microscope: C, J, M - ×100, the rest - ×400

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2. Fig. 2. Chromatogram (HPLC/UV) of 50% alcohol extract of anti-inflammatory collection: 1 - arbutin, 2 - gallic acid, 3 - bergenein, 4 - chlorogenic acid, 5 - rutin, 6 - rosmarinic acid. Notes: Abscissa axis - retention time, ordinate axis - peak intensity

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3. Fig. 3. Differential spectra of luteolin (1) and anti-inflammatory collection (2) with the addition of 2% aluminum chloride solution. Abscissa axis - wavelength, nm; ordinate axis - optical density

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