Influence of a new biostimulant and hydrothermal conditions on the dynamics of phyloquinone accumulation in leaves of Urtica Dioica L.

Introduction. In connection with the development of agricultural and medicinal plant growing, the search for effective biostimulants is relevant. The results of long-term research by the authors showed that the product of paracetamol biodegradation, obtained from paracetamol waste by biotechnological means, is an active stimulator of plant growth and an inducer of accumulation of biologically active substances in them.

The aim of this study is to investigate the effect of paracetamol biodegradation product and hydrothermal conditions of vegetation periods of different years on the dynamics of phylloquinone (vitamin K1) accumulation in leaves of Urtica dioica L.

Material and methods. The work used the biodegradation product of paracetamol as a biostimulator, and the plant growth stimulator drug "Zircon" as a reference when conducting field experiments in places of natural growth of nettles in the Perm Territory in 2022 and 2023. In the plant raw materials collected from the experimental and control sites at the beginning and end of the vegetation phase, the phylloquinone content was determined by high-performance liquid chromatography. The hydrothermal coefficient was used to assess the effect of hydrothermal conditions of the growing season on the accumulation of phylloquinone.

Results. Under the influence of the biostimulator used in the work, the phylloquinone content in nettle leaves at the beginning of the 2022 vegetation phase increased by 12.0% compared to the control, by 26.6% at the end of the phase, and by 2.6% and 9.1%, respectively, when treated with Zircon. The increase in phylloquinone content at the beginning and at the end of the 2023 vegetation phase under the influence of PBP was 1.5% and 6.5%, and when treated with Zircon – 0.4% and 0.6%, respectively. More favorable hydrothermal conditions compared to 2023 contributed to greater accumulation of phylloquinone in the control and experimental samples of 2022.

Conclusion. The product of paracetamol biodegradation showed properties of an inducer of phylloquinone accumulation in stinging nettle leaves. A significant effect of hydrothermal conditions of the growing environment on the accumulation of phylloquinone by this plant in the vegetation periods of different years was shown.