Polyphenolic composition and antiproliferative activity of small malt bean extract (Glycyrrhiza glabra L.)
- Authors: Gudinskaya N.I1, Nikolaev A.A1
-
Affiliations:
- Astrakhan State Medical University of the Ministry of Health of the Russian Federation
- Issue: Vol 24, No 11 (2021)
- Pages: 15-19
- Section: Articles
- URL: https://journals.eco-vector.com/1560-9596/article/view/112895
- DOI: https://doi.org/10.29296/25877313-2021-11-03
- ID: 112895
Cite item
Abstract
Relevance. In recent years, there has been increased interest in finding and using natural, highly effective and inexpensive plant antioxidants to replace existing synthetic antioxidants. Dried licorice roots have long been widely used as a food flavoring and for various medicinal purposes. Licorice root preparations are known for their significant antioxidant, anti-inflammatory and antiproliferative properties. However, the antiproliferative activity of polyphenols from licorice beans has not been extensively studied. The aim of this study was to study the composition of polyphenols in licorice beans and their antiproliferative activity. Material and methods. We used 1000 g of dried mass of licorice beans. Chemicals and reagents used in the work were of analytical grade. We used an ethanol extract of licorice beans. The analysis of polyphenols in ethanol extract of licorice beans (EEBS) was carried out on the basis of the Folin - Ciocalteu method. The content of polyphenols was expressed in grams of gallic acid equivalent (EGA) per 100 g dry weight (g EGA / 100 g DM). Polyphenols were identified by HPLC (Gilson C18 column 4.6x250 mm, 5 jim). Cytotoxicity analysis was performed on LNCaP cell culture. After 48 h of exposure to EEBS, a standard MTT test was performed. Results. The total content of polyphenols in the EEBS in recalculation of gallic acid was 37.17 jig. Glabrin, catechin, rutin, lycochalcon A, and ellagic acid have been identified. After an incubation period of 48 hours, the ethanolic licorice bean extract showed a dose-dependent inhibition of cell growth from 40 to 140 |jg/ml. The maximum effect was obtained from 100 |jg/ml, which demonstrates the cytotoxic effect of EEBS on LNCaP cells over 85%. Conclusion. It has been shown that licorice beans, along with licorice root, can be used as a powerful biologically active source of natural antioxidants and anticancer agents.
Full Text
![Restricted Access](https://journals.eco-vector.com/lib/pkp/templates/images/icons/text_lock.png)
About the authors
N. I Gudinskaya
Astrakhan State Medical University of the Ministry of Health of the Russian FederationPh.D. (Med.), Associate Professor
A. A Nikolaev
Astrakhan State Medical University of the Ministry of Health of the Russian FederationDr.Sc. (Med.), Professor
References
- Ferrazzano G.F., Amato I., Ingenito A., Zarrelli A., Pinto G., Pollio A. Plant polyphenols and their anti-cariogenic properties: Areview. Molecules. 2011; 16: 1486-1507.
- Grenier D., Marcoux E., Azelmat J., A Ben Lagha P. Gauthier Biocompatible combinations of nisin and licorice polyphenols exert synergistic bactericidal effects against Enterococcus faecalis and inhibit NF-kB activation in monocytes. AMB Express. 2020; 10(1): 120-129.
- Fraga C.G., Croft K.D., Kennedy D.O., Tomas-Barberan F.A. The effects of polyphenols and other bioactives on human health. Food Funct. 2019; 10: 514-528.
- Daglia M. Polyphenols as antimicrobial agents. Curr. Opin. Biotechnol. 2012; 23: 174-181.
- Vlaisavljevic S., Sibul F., Sinka I., Zupko I., Ocsovszki I., Jovanovic-Santa S. Chemical composition, antioxidant and anticancer activity of licorice from Fruska Gora locality. Ind. Crop. Prod. 2018; 112: 217-224.
- Гаммерман А.Ф. Лекарственные растения. М.: Высшая школа. 990. 453 с.
- Fogarasi, Kun, Tanko, Stefanovits-Banyai, Hegyesne-Vecseri. A comparative assessment of antioxidant properties, total phenolic content of einkorn, wheat, barley and their malts. Food Chem. 2015; 167(1): 1-6. doi: 10.1016/j.food-chem.2014.06.084. Epub 2014 Jul 1.
- Zhang Y., Zhou X., Tao W., Li L., Wei C., Duan J. Antioxidant and antiproliferative activities of proanthocyanidins from Chinese bayberry (Myrica rubra Sieb. et Zucc. leaves. Journal of Functional Foods. 2016; 27: 645-654.
Supplementary files
![](/img/style/loading.gif)