Selection and evaluation of conservation conditions of Claviceps purpurea saprophytic lines
- Authors: Volnin A.A.1, Bobyleva R.I.1, Tsybulko N.S.1, Savin P.S.1, Shtrom K.A.2, Myasnikova S.B.1, Bokhan A.I.1
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Affiliations:
- All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
- MIREA – Russian Technological University (Moscow State University of Fine Chemical Technologies named after Lomonosov)
- Issue: Vol 27, No 8 (2024)
- Pages: 50-58
- Section: Biological chemistry
- URL: https://journals.eco-vector.com/1560-9596/article/view/635311
- DOI: https://doi.org/10.29296/25877313-2024-08-07
- ID: 635311
Cite item
Abstract
Introduction. Ergot Claviceps purperea plays an important role in health-care technologies. Accumulation of genetic resources of industrial pharmaceutical strains of parasitic ergot is the actual scientific problem. This work is devoted to development of selection and evaluation of conservation conditions of Claviceps purpurea saprophytic lines.
Conidiospores and saprophytic mycelium were frozen at –20 0C, after 10 months of storage and cultivated in Erlenmayer flasks on a liquid medium and on an agarised medium. Cryopreserved samples of conidiospores and mycelium retained the ability to grow and alkaloids biosynthesis in vitro.
Results. The best lines were selected from sclerotias of 3 parasitic strains and deposited in the form of axenic and saprophytic mycelium for long-term storage (ergotoxin ВКМ-F-2450-D-23-1, ergotoxin ВКМ-F-2450-D-23-9, ergotamine ВКМ- F-2641-D-S-2, ergocornam ВКМ-F-3662-D-22-5).
Sclerotia of ergotamine-producing strain had a level of alkaloid synthesis of 0.78 g/100 g, with an ergotamine content of 67.9%, and demonstrated of pigmented purple mycelium in axenic culture. Sclerotias of ergotoxin-producing strain – 0.76 and 0.59 g/100 g, containing 51.5 and 50.3% of the sum of ergocornine and α-ergocryptine, 20 and 22% of β-ergocryptine, respectively, both lines demonstrated sclerotia-like mycelium and alkaloids biosynthesis in saprophytic cultivation on agarised medium. Sclerotia of ergocornam-producing strain – 0.79 g/100 g, containing 18.2% of ergocornam and 24.6% of ergometrine, demonstrated pigmented purple mycelium in axenic culture.
Conclusion. Development of long-term storage biocollections, including cryocollections for organisms producing pharmaceuticals raw substances, creates the basis for technological sovereignty of the Russian Federation. It is reducing dependence on imported drugs and pharmaceuticals raw substances, including ergoalkaloids.
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About the authors
A. A. Volnin
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Author for correspondence.
Email: volnin.a@mail.ru
ORCID iD: 0000-0001-9222-536X
Ph.D. (Biol.), Leading Research Scientist, Laboratory of Biotechnology
Russian Federation, 7, ul. Grina, Moscow, 117216R. I. Bobyleva
Email: volnin.a@mail.ru
к.б.н.
Russian FederationN. S. Tsybulko
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: volnin.a@mail.ru
ORCID iD: 0000-0001-9542-5017
Ph.D. (Pharm.), Engineer, Laboratory of Biotechnology
Russian Federation, 7, ul. Grina, Moscow, 117216P. S. Savin
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: volnin.a@mail.ru
ORCID iD: 0000-0002-5441-3471
Ph.D. (Biol.), Leading Research Scientist, Laboratory of Biotechnology
Russian Federation, 7, ul. Grina, Moscow, 117216K. A. Shtrom
MIREA – Russian Technological University (Moscow State University of Fine Chemical Technologies named after Lomonosov)
Email: volnin.a@mail.ru
Master
Russian Federation, Vernadsky Avenue, 86, Moscow, 119454S. B. Myasnikova
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: volnin.a@mail.ru
ORCID iD: 0000-0002-6318-2433
Research Scientist, Laboratory of Biotechnology
Russian Federation, 7, ul. Grina, Moscow, 117216A. I. Bokhan
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: volnin.a@mail.ru
ORCID iD: 0000-0003-4154-3709
Dr.Sc. (Agrical.), Head of Laboratory of Biotechnology
Russian Federation, 7, ul. Grina, Moscow, 117216References
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