The amino acid composition of Phlojodicarpus sibiricus (Steph. ex Spreng.) Koso-Pol

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Abstract

Introduction. Phlojodicarpus sibiricus is a valuable plant raw material – a source of coumarins, on the basis of which effective dosage forms (dry extracts, tinctures) for the treatment of neurodegenerative diseases have been created. The chemical composition of the plant is well studied, but there is no information about the composition of amino acids in both aboveground and underground parts of plants.

The aim of the study is to determine amino acid composition and their content in the herbs, roots and rhizomes of Phlojodicarpus sibiricus from different habitats.

Material and methods. The object of the study are herbs, roots and rhizomes of Phlojodicarpus sibiricus, collected in 2020, 2022, and 2023 during the fruiting phase from different populations in the Republic of Buryatia and Trans-Baikal Territory. Analytical samples were obtained by the quartering method. The amino acid composition and content were determined according Russian National Standard R 55569-2013 using the capillary electrophoresis system "Kapel-105".

Results. 20 amino acids have been found in grass and rhizomes and roots, of which 8 are essential. The total content of 20 amino acids in the underground organs of plants was in the range of 31,62–48,80 mg/g of dry weight, of which the share of essential acids accounted for an average of up to 23% (about 39 mg/g). In the herb, these values were up to 2,5 times higher than in the rhizomes and roots.

Conclusion. In this regard, we have studied for the first time the composition and amino acid content of grass, rhizomes and roots of Siberian bulbous fruit from different populations of the Republic of Buryatia and the Trans-Baikal region. The obtained results supplement the existing information on the chemical composition of valuable raw materials and can be used in the future for development of medicines and biologically active suplements based on Phlojodicarpus sibiricus.

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About the authors

Yu. N. Nikolaev

Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences; V.R. Filipov Buryat State Agricultural Academy

Author for correspondence.
Email: yuriynik361@gmail.com
ORCID iD: 0009-0000-1932-3289
SPIN-code: 3068-6395

Post-graduate Student, Research Assistant

Russian Federation, Sakhyanovoy str., 6, Ulan-Ude, 670047, Republic of Buryatia; Pushkina str. 8, Ulan-Ude, 670024, Republic of Buryatia

A. S. Taraskina

Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences; Dorji Banzarov Buryat State University

Email: astaraskina@mail.ru
ORCID iD: 0000-0002-1341-294X
SPIN-code: 5325-8204

Ph.D. (Pharm.), Research Scientist, Master's Student

Russian Federation, Sakhyanovoy str., 6, Ulan-Ude, 670047, Republic of Buryatia; Smolina str. 24 "a", Ulan-Ude, 670000, Republic of Buryatia

S. M. Gulyaev

Institute of General and Experimental Biology of the Siberian Branch of the Russian Academy of Sciences

Email: s-gulyaev@inbox.ru
ORCID iD: 0000-0001-8412-5799
SPIN-code: 5084-6499

Ph.D. (Med.), Senior Research Scientist

Russian Federation, Sakhyanovoy str., 6, Ulan-Ude, 670047, Republic of Buryatia

V. A. Achituev

V.R. Filipov Buryat State Agricultural Academy

Email: s-gulyaev@inbox.ru
ORCID iD: 0009-0002-9025-8701
SPIN-code: 8286-3210

Ph.D. (Agric.), Dean of the Faculty of Technology

Russian Federation, Pushkina str. 8, Ulan-Ude, 670024, Republic of Buryatia

Zh. A. Tykheev

Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences; V.R. Filipov Buryat State Agricultural Academy

Email: gagarin199313@gmail.com
ORCID iD: 0000-0002-6389-2494
SPIN-code: 2273-9720

Ph.D. (Pharm.), Head of the Laboratory, Senior Research Scientist

Russian Federation, Sakhyanovoy str., 6, Ulan-Ude, 670047, Republic of Buryatia; Pushkina str. 8, Ulan-Ude, 670024, Republic of Buryatia

V. V. Taraskin

Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences; V.R. Filipov Buryat State Agricultural Academy

Email: vvtaraskin@binm.ru
ORCID iD: 0000-0003-0909-2629
SPIN-code: 4879-1028

Ph.D. (Pharm.), Head of the Laboratory, Senior Research Scientist

Russian Federation, Sakhyanovoy str., 6, Ulan-Ude, 670047, Republic of Buryatia; Pushkina str. 8, Ulan-Ude, 670024, Republic of Buryatia

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