Development and validation of a method for quantitative determination of the amount of fructosans in chicory roots
- Authors: Gulenkov А.S.1, Kulyak O.Y.1,2, Dul V.N.1, Mavrina P.O.1, Zotov I.E.3, Tomilina A.Y.3, Mizina P.G.1
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Affiliations:
- All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
- Lomonosov Moscow State University
- D. Mendeleev University of Chemical Technology of Russia
- Issue: Vol 28, No 8 (2025)
- Pages: 27-37
- Section: Pharmaceutical chemistry
- URL: https://journals.eco-vector.com/1560-9596/article/view/689303
- DOI: https://doi.org/10.29296/25877313-2025-08-04
- ID: 689303
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Abstract
Introduction. To develop regulatory documentation for raw materials of chicory roots, it is necessary to standardize the raw materials according to the content of the main active substance, which is fructosans (the main substance is inulin) [1, 2]. The Russian Pharmacopoeia 15th edition, includes articles ФС.2.5.0070.18, ФС.2.5.0025.15, which include a method for determining the amount of fructosans in a plant, based on the spectrophotometric determination of the absorption of a colored complex of acid hydrolysis products of fructosans - 5-hydroxymethylfurfural (5-HMF) with resorcinol. However, in the scientific literature there are methods based on determining the absorption of hydrolysis products without additional complex formation.
The aim – development and validation of a method for the quantitative determination of the amount of fructosans in terms of inulin in chicory roots with spectrophotometric determination of 5-HMF absorption, without the use of additional reagents (resorcinol, thiourea).
Material and methods. The object of the study is the roots of common chicory. The development and validation of the method for the quantitative determination of the sum of fructosans in terms of inulin was carried out by spectrophotometry.
Results. The developed method has been positively evaluated in the following parameters: specificity (coincidence of absorption maxima at a wavelength of (286±2) nm); linearity (correlation coefficient 0.9987); accuracy (range of recovery percentage – from 96.59% to 103.50% and has an average value of 99.97%); repeatability (coefficient of variation (RSD) 1.52% < 2.00%); reproducibility (coefficients of variation of intralaboratory reproducibility do not exceed 2%).
Conclusion. The obtained results allow us to consider the developed method suitable for reliable quantitative determination of the amount of fructosans in terms of inulin in the roots of common chicory.
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About the authors
А. S. Gulenkov
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Author for correspondence.
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0002-1532-9330
SPIN-code: 9436-3210
Ph.D. (Pharm), Leading Researcher, Department of Chemistry of Natural Compounds
Russian Federation, 7 Grin str., Moscow, 117216O. Yu. Kulyak
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants; Lomonosov Moscow State University
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0003-0385-3474
SPIN-code: 9697-1220
Ph.D. (Pharm), Leading Researcher, Department of Chemistry of Natural Compounds, Head of the Testing Center, Associate Professor of the Department of Pharmaceutical Chemistry and Organization of Pharmaceutical Affairs of the Medical Scientific and Educational Institute
Russian Federation, 7 Grin str., Moscow, 117216; Leninskie Gory 1, Moscow, 119991V. N. Dul
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0001-7824-4417
SPIN-code: 8192-5803
Ph.D. (Pharm.), Leading Researcher, Department of Chemistry of Natural Compounds
Russian Federation, 7 Grin str., Moscow, 117216P. O. Mavrina
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0002-8559-8771
SPIN-code: 7119-8667
Post-graduate Student, Junior Researcher, Department of Chemistry of Natural Compounds
Russian Federation, 7 Grin str., Moscow, 117216I. E. Zotov
D. Mendeleev University of Chemical Technology of Russia
Email: gulenkov@vilarnii.ru
ORCID iD: 0009-0005-0705-8672
Student of the Faculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, Department of Chemistry and Technology of Biomedical Drugs
Russian Federation, Miusskaya square 9, Moscow, 125047A. Yu. Tomilina
D. Mendeleev University of Chemical Technology of Russia
Email: gulenkov@vilarnii.ru
ORCID iD: 0009-0009-7751-4310
Student of the Faculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, Department of Chemistry and Technology of Biomedical Drugs
Russian Federation, Miusskaya square 9, Moscow, 125047P. G. Mizina
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: gulenkov@vilarnii.ru
ORCID iD: 0000-0001-6510-9603
SPIN-code: 7084-1962
Dr.Sc. (Pharm.), Professor, Advisor to the Director, Honored Scientist of the Russian Federation
Russian Federation, 7 Grin str., Moscow, 117216References
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