INFLUENCE OF SUCCINATE ON SOME INDICATORS OF BIOENERGY METABOLISM IN SEMINAL VESICLES AND EPIDIDYMIS IN MALE RATS UNDER CONDITIONS OF CHRONIC HYPOXIA

Relevance. Lactate in the cell performs a number of functions in the regulation of metabolism and is involved in energy metabolism. Under conditions of hypoxia, cells switch to succinate oxidation to maintain energy metabolism. The mechanism of action of succinate as an effective antihypoxic agent has been little studied, but its effectiveness is beyond doubt. Objective. Evaluation of changes in lactate level and LDH activity under conditions of chronic hypoxia and during the use of succinate. Material and methods. We used a model of chronic hypoxia in the modification: the animals were placed in a sealed chamber with a volume of 1.2 liters, connected to a gas analyzer, and were there until the oxygen content in the air reached 10%. The experiment was repeated daily for 14 days. The introduction of succinic acid was carried out in the form of a 4% solution in 0.9% NaCl intraperitoneally for 14 days at a dose of 100 mg / kg. Determined lactic acid and LDH activity in the cytoplasmic and mitochondrial fractions of seminal vesicles and epididymis. Results. Chronic normobaric hypoxia leads to a significant decrease in LDH activity in the epididymis head both in the cytoplasmic and mitochondrial fractions, and to a decrease in LDH activity in the epididymis tail only in the cytoplasm. In the seminal vesicles, no statistically significant changes in LDH activity were found under conditions of chronic hypoxia. The introduction of succinate against the background of hypoxia led to an increase in LDH activity relative to the group with hypoxia of the cytoplasmic fraction in the tail and the mitochondrial fraction of the epididymis head. The accumulation of lactate in the mitochondria of the epididymis head and in the cytoplasm of the seminal vesicles was also noted. Conclusions. Chronic normobaric hypoxia leads to inhibition of LDH activity. The accumulation of lactic acid occurs with an increase in the LDH activity of the mitochondrial fraction in comparison with the LDH activity of the cytoplasm. The receipt of succinate by animals during chronic hypoxia led to an increase in lactate in the mitochondrial fraction and facilitated adaptation of the mitochondrial function.

chronic hypoxia, succinic acid (succinate), lactic acid (lactate), lactate dehydrogenase, seminal vesicles, epididymis