Choleretic effect of the Hedysarum alpinum L. extract

Relevance. The topicality of the studies is driven by the increasing interest of the population of the plant medicinal remedies and needs in the replenishment of the national plant preparations list for prevention and treatment of the diseases.

The aim of the present study was to evaluate the choleretic activity of the Hedysarum alpinum L. extract.

Material and methods. The experiments were carried out on 40 white male Wistar rats weighting 170.0–200.0 g.

In the conditions of acute experiments, the choleretic effect of the extract was evaluated in rats anesthetized by thiopental sodium (40 mg/kg, intraperitoneally) taking into account the whole volume of the secreted bile, contents of the bile acids, bilirubin and cholesterol. The bile was collected by the cannula inserted into the common bile duct for 4 hours. The Hedysarum alpinum extract obtained using the alcohol-water extraction from the plant material gathered in the Baikal region in 2021–2022 and conforming to the temporary pharmacopeial monograph 42-1998-85 was introduced into the duodenum of experimental animals (group 2, 3, 4, 5) at the doses 50–300 mg/kg in the form of water solution. Each group consisted of 8 animals. The control group (group 1) received the purified water in the same volume.

Results. The studies have shown that the Hedysarum alpinum extract stimulates the choleretic reaction, hastening the bile secretion and increasing the content of the bile acids in the secreted bile. Dose escalation of the Hedysarum alpinum extract has shown the further increase in the bile secretion and the content of bile acids as well as bilirubin and cholesterol in the secreted bile. The statistical data processing was made with the use of the Statistica application program.

Conclusion. The Hedysarum alpinum dry extract in experimental-therapeutic doses has the marked choleretic effect in anesthetized while rats.