Influence of Lophanthus chinensis dry extract on the morphofunctional state of Wistar rats the brain during long-term alcoholization

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

The study purpose – to evaluate the effect of the Lophanthus chinensis dry extract on the Wistar rats brain morphofunctional state against the background of long-term alcoholization.

Material and methods. Alcohol intoxication was induced by long-term administration (6 weeks) of 40% ethanol in rats. Lophanthus chinensis dry extract at a dose of 100 mg/kg was administered from the 3rd week of the experiment. On days 42-45, the animals were tested in an "open field", an elevated plus maze, and they developed a conditioned passive avoidance reaction. After which a neurons pathomorphological study in the cerebral cortex was carried out.

Results. It has been established that L. chinensis dry extract exhibits a neuroprotective effect during long-term alcohol intoxication, preventing the development of structural disorders in the cerebral cortex, thereby reducing anxiety and emotionality in animals, enhancing orientation-exploratory activity and normalizing mnestic functions.

Conclusion. L. chinensis dry extract has a neuroprotective effect during long-term alcohol intoxication.

全文:

受限制的访问

作者简介

Ya. Razuvaeva

Institute of General and Experimental Biology SB RAS

编辑信件的主要联系方式.
Email: tatur75@mail.ru

Dr.Sc. (Biol.), Senior Research Scientist

俄罗斯联邦, Ulan-Ude, Republic of Buryatia

E. Khaltagarova

Institute of General and Experimental Biology SB RAS; Banzarov Buryat State University

Email: haltik@yandex.ru

Post-graduate Student, Lecturer

俄罗斯联邦, Ulan-Ude, Republic of Buryatia; Ulan-Ude, Republic of Buryatia

А. Toropova

Institute of General and Experimental Biology SB RAS

Email: anyuta-tor@mail.ru

Ph.D. (Biol.), Senior Research Scientist

俄罗斯联邦, Ulan-Ude, Republic of Buryatia

К. Markova

Institute of General and Experimental Biology SB RAS

Email: kristen_kartland@mail.ru

Ph.D. (Med.), Senior Research Scientist

俄罗斯联邦, Ulan-Ude, Republic of Buryatia

D. Olennikov

Institute of General and Experimental Biology SB RAS

Email: tatur75@mail.ru

Dr.Sc. (Pharm.), Head laboratory

俄罗斯联邦, Ulan-Ude, Republic of Buryatia

参考

  1. Global, regional, and national age-sex-specific mortality for 282 causes of death in 195 countries and territories, 1980–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet. 2018; 392: 1736–1788. doi: 10.1016/S0140-6736 (18)32203-7.
  2. Singal A.K., Mathurin P. Diagnosis and treatment of alcohol-associated liver disease: A review. JAMA. 2021; 326(2): 165–176. doi: 10.1001/jama.2021.7683.
  3. Yoshiji H., Nagoshi S., Akahane T., et al. Evidence-based clinical practice guidelines for liver cirrhosis. J. Gastroenterol. 2020; 56: 593–619. doi: 10.1007/s00535-021-01788-x
  4. Tranah T.H., Paolino A., Shawcross D.L. Pathophysiological mechanisms of hepatic encephalopathy. Clin. Liver Dis. (Hoboken). 2015; 5(3): 59–63. doi: 10.1002/cld.445.
  5. Ilchenko L.Yu., Nikitin I.G. Hyperammonemia in patients at the pre-cirrhotic stage: a clinical reality? Archives of Internal Medicine. 2018; 8(3): 186–193. doi: 10.20514/2226-6704-2018-8-3-186-193.
  6. Kornerup L.S., Gluud L.L., Vilstrup H., Dam G. Update on the therapeutic management of hepatic encephalopathy. Curr. Gastroenterol. Rep. 2018; 20(5): 21. doi: 10.1007/s11894-018-0627-8.
  7. Aseeva T.A., Dashiev D.B., Dashiev A.D. i dr. Tibetan medicine among the Buryats. – Novosibirsk: Publishing House SB RAS. 2008; 324 p.
  8. Batorova S.M., Yakovlev G.P., Aseeva T.A. Directory of medicinal plants of traditional Tibetan medicine. Novosibirsk: Nauka, 2013. –292 p.
  9. Brun E.A., Polunina A.G. Depression, anxiety disorders and antisocial personality disorder as risk factors or consequences of alcoholism and drug addiction: gender aspects. Narcology. 2018; 11: 74–85. doi: 10.25557/1682-8313.2018.11.74-85.
  10. Guidelines for conducting preclinical studies of drugs. Part one. Ed. A.N. Mironov. M.: Grif and K. 2012; 944.
  11. Microscopic technique: Guide. Ed. D.S. Sarkisova and Yu.L. Perova. M.: Medicine. 1996; 544 p.
  12. Olennikov D.N., Chirikova N.K., Tankhaeva L.M. Chemical study of Lophanthus chinensis. Chemistry of natural compounds. 2010; 46 (2): 301–302. doi: 10.1007/s10600-010-9596-3.
  13. Olennikov D.N., Tankhaeva L.M., Rokhin A.V. Lamiaceae carbohydrates. VI. water-soluble polysaccharides from Lophanthus chinensis. Chemistry of natural compounds. 2009; 45(3): 300–303. doi: 10.1007/s10600-009-9358-2.
  14. Tanaka N., Yamada K., Shimomoto Y., et al. Lophachinins A-E, abietane diterpenes from a Mongolian traditional herbal medicine Lophanthus chinensis. Fitoterapia. 2020; 146: 104702. doi: 10.1016/j.fitote.2020.104702.
  15. Han Y., Zhang T., Su J., et al. Apigenin attenuates oxidative stress and neuronal apoptosis in early brain injury following subarachnoid hemorrhage. J. Clin. Neurosci. 2017; 40: 157–162. doi: 10.1016/j.jocn.2017.03.003.
  16. Liang G., Shi B., Luo W., Yang J. The protective effect of caffeic acid on global cerebral ischemia-reperfusion injury in rats. Behavioral and Brain Functions. 2015; 11: 18. doi: 10.1186/s12993-015-0064-x.
  17. Nabavi S.F., Braidy N., Gortzi O., et al. Luteolin as an anti-inflammatory and neuroprotective agent: A brief review. Brain Res. Bull. 2015; 119 (PtA): 1–11. doi: 10.1016/j.brainresbull.2015.09.002.
  18. Venigalla M., Gyengesi E., Münch G. Curcumin and Apigenin – novel and promising therapeutics against chronic neuroinflammation in Alzheimer's disease. Neural. Regen. Res. 2015; 10(8): 1181–1185. doi: 10.4103/1673-5374.162686.
  19. Xu J., Wang H., Ding K., et al. Luteolin provides neuroprotection in models of traumatic brain injury via the Nrf2-ARE pathway. Free Radic. Biol. Med. 2014; 71: 186–195. doi: 10.1016/j.freeradbiomed.2014.03.009.

补充文件

附件文件
动作
1. JATS XML
下载
2. Fig. 1. Behavioral indicators of Wistar rats in the "open field" against the background of long-term alcoholism

下载 (152KB)
索引源数据
3. Fig. 2. Behavioral indicators of Wistar rats in the plus maze against the background of long-term alcoholism

下载 (152KB)
索引源数据
4. Fig. 3. Duration of stay of Wistar rats in the compartments of the elevated plus maze against the background of long-term alcoholization

下载 (139KB)
索引源数据
5. Fig. 4. Duration of the latent period in Wistar rats in the CPAR test against the background of long-term alcoholization (hereinafter and everywhere * - the results are statistically significant in relation to the control at p≤0.5)

下载 (195KB)
索引源数据
6. Fig. 5. Number of animals with a developed conditioned reflex against the background of long-term alcoholism

下载 (297KB)
索引源数据
7. Fig. 6. Types of neurons in the cerebral cortex in Wistar rats against the background of long-term alcoholism

下载 (239KB)
索引源数据

版权所有 © Russkiy Vrach Publishing House, 2024
##common.cookie##