Effect of acetone extract of Agrimonia Pilosa Ledeb. Leaves on amylase and lipase

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Introduction. In recent years, digestive enzymes for targeted action on metabolic pathways have attracted considerable interest. Medicinal plants are rich in various biologically active compounds and, compared to synthetic inhibitors, exhibit lower toxicity as inhibitory ligands of gastrointestinal enzymes. In food practice, the range of applications of Agrimonia pilosa Ledeb. leaf extracts may expand.

Objective. The purpose of the present investigation was aimed at studying the content of phenolic compounds including flavonoids in the A. pilosa leaf acetone extract followed by its influence on the activity of digestive enzymes (amylase and lipase).

Material and methods. In vitro methods using artificial substrates synthesized for specific interaction with lipase and amylase were used to determine the activity of pancreatic enzymes.

Results. Our results showed that the acetone extract of A. pilosa leaves enriched in polyphenolic compounds inhibited the activity of digestive enzymes in vitro models. Inhibition of amylase was more significant than lipase.

Conclusions. If the effect of A. pilosa on lipase has previously been the subject of study, according to literature data, then, to our knowledge, no such studies have been conducted on amylase, giving the results obtained novelty and opening up prospects for further research in this direction.

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作者简介

E. Sokolova

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

编辑信件的主要联系方式.
Email: eka9739@yandex.ru
ORCID iD: 0000-0002-7605-9688
SPIN 代码: 3578-1374

Ph.D. (Biol.), Leading Research Scientist

俄罗斯联邦, 7 Grina str., Moscow, 117216

T. Krol

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Email: tatianakroll1@gmail.com
ORCID iD: 0000-0003-4642-651X
SPIN 代码: 1620-2290

Ph.D. (Agric.), Head of the Laboratory of Genomics and Biochemistry of Medicinal Plants

俄罗斯联邦, 7 Grina str., Moscow, 117216

D. Baleev

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Email: dbaleev@gmail.com
ORCID iD: 0000-0002-1228-0594
SPIN 代码: 5487-2926

Ph.D. (Agric.), Head of the Laboratory of Atomic-Molecular Bioregulation and Selection

俄罗斯联邦, 7 Grina str., Moscow, 117216

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2. Fig. 1. Activity of digestive enzymes lipase and amylase in the presence of acetone extract of Agrimonia pilosa Ledeb. leaves inin vitro models (* – significance statistical difference relative to the blank, p≤ 0.05)

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