Development of Autistic Spectrum Symptoms under Chronic Social Defeat Stress in Anxious Male Mice: Effects of Diazepam
- Authors: Kovalenko I.L.1, Kudryavtseva N.N.1, Kovalenko IL2, Kudryavtseva NN2
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Affiliations:
- Institute of Cytology and Genetics SD RAS
- Issue: Vol 10, No 1-2 (2010)
- Pages: 2624-2635
- Section: Articles
- URL: https://journals.eco-vector.com/1606-8181/article/view/322019
- DOI: https://doi.org/10.17816/phbn322019
- ID: 322019
Cite item
Full Text
Abstract
Kovalenko IL, Kudryavtseva NN. [Development of autistic spectrum symptoms under chronic social defeat stress in anxious male mice: effects of diazepam]. Psychopharmacol Biol Narcol. 2010;10(1-2):2624-2635.
Epub 23 July, 2010. Russian.
BACKGROUND. It is well known that psychoemotional disorders may be accompanied by decreased sociability in humans. It has been shown that repeated social defeat stress produced by 10 daily agonistic interactions led to development of the pronounced anxiety in male mice. The paper aimed to study the features of communicative behavior in anxious animals.
METHODS. The sensory contact model was used to form high level of anxiety in male mice. The level of anxiety was measured in elevated plus-maze test. Communicative behavior was studied in the social interaction and partition tests as a behavioral reaction toward the other partner.
RESULTS. It has been shown that communication toward familiar and unfamiliar partners and exploratory activity were dramatically decreased in anxious mice. Avoidance of approaching partner and demonstration of self-grooming were significantly increased in animals under repeated social defeats. Chronic diazepam treatment (0.5 mg/kg, i.p., 2 weeks) led to significant decrease of anxiety level and to improvement of communication. Diazepam completely has restored the level of sociability and exploratory activity and reduced avoidance behavior of approaching partner. Diazepam did not affect self-grooming behavior.
CONCLUSIONS. Main factor which provokes impairment of communication in defeated male mice is the high level of anxiety. Results have demonstrated similarity between changes in social behaviors in anxious animals and symptoms of autistic spectrum disorders in humans. [in Russian with English Summary].
Date submitted: June 06, 2010; Accepted: July 22, 2010.
Citation: Psychopharmacol Biol Narcol. 2010;10 (1-2):2624-2635
Epub 23 July, 2010. Russian.
BACKGROUND. It is well known that psychoemotional disorders may be accompanied by decreased sociability in humans. It has been shown that repeated social defeat stress produced by 10 daily agonistic interactions led to development of the pronounced anxiety in male mice. The paper aimed to study the features of communicative behavior in anxious animals.
METHODS. The sensory contact model was used to form high level of anxiety in male mice. The level of anxiety was measured in elevated plus-maze test. Communicative behavior was studied in the social interaction and partition tests as a behavioral reaction toward the other partner.
RESULTS. It has been shown that communication toward familiar and unfamiliar partners and exploratory activity were dramatically decreased in anxious mice. Avoidance of approaching partner and demonstration of self-grooming were significantly increased in animals under repeated social defeats. Chronic diazepam treatment (0.5 mg/kg, i.p., 2 weeks) led to significant decrease of anxiety level and to improvement of communication. Diazepam completely has restored the level of sociability and exploratory activity and reduced avoidance behavior of approaching partner. Diazepam did not affect self-grooming behavior.
CONCLUSIONS. Main factor which provokes impairment of communication in defeated male mice is the high level of anxiety. Results have demonstrated similarity between changes in social behaviors in anxious animals and symptoms of autistic spectrum disorders in humans. [in Russian with English Summary].
Date submitted: June 06, 2010; Accepted: July 22, 2010.
Citation: Psychopharmacol Biol Narcol. 2010;10 (1-2):2624-2635
About the authors
Irina Leonidovna Kovalenko
Nataliya Nikolaevna Kudryavtseva
Email: natnik@bionet.nsc.ru
I L Kovalenko
Institute of Cytology and Genetics SD RAS; Institute of Cytology and Genetics SD RAS
N N Kudryavtseva
Institute of Cytology and Genetics SD RAS
Email: natnik@bionet.nsc.ru
; Institute of Cytology and Genetics SD RAS
References
- Happ F., Ronald A. The "fractionable autism triad": a review of evidence from behavioural, genetic, cognitive and neural research. Neuropsychol Rev. 2008; 18(4): 287-304.
- Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, Text Revision: DSM-IV-TR, by the American Psychiatric Association, 943 pp, paper, ISBN 0-89042-025-4, Washington, DC, American Psychiatric Association, 2000.
- Abrahams B.S., Geschwind D.H. Advances in autism genetics: on the threshold of a new neurobiology. Nat Rev Genet. 2008; 9(5): 341-355.
- Cook E.H. Genetics of autism. Child Adolesc Psychiatr Clin N Am. 2001; 10: 333-350.
- Rapin I., Tuchman R.F. Autism: definition, neurobiology, screening, diagnosis. Pediatr Clin North Am. 2008; 55(5): 1129-46.
- Newschaffer C.J., Croen L.A., Daniels J., Giarelli E., Grether J.K., Levy S.E., Mandell D.S., Miller L.A., Pinto-Martin J., Reaven J., Reynolds A.M., Rice C.E., Schendel D., Windham G.C. The epidemiology of autism spectrum disorders. Annu Rev Public Health. 2007; 28: 235-258.
- Brodkin E.S. BALB/c mice: low sociability and other phenotypes that may be relevant to autism. Behav Brain Res. 2007; 176(1): 53-65.
- Crawley J.N. Mouse behavioral assays relevant to the symptoms of autism. Brain Pathol. 2007; (4): 448-459.
- Moy S.S., Nadler J.J., Young N.B., Nonneman R.J., Grossman A.W., Murphy D.L., D'Ercole A.J., Crawley J.N., Magnuson T.R., Lauder J.M. Social approach in genetically engineered mouse lines relevant to autism. Genes Brain Behav. 2009; 8(2): 129-142.
- Kudryavtseva N.N. Use of the "partition" test in behavioral and pharmacological experiments. Neurosci Behav Physiol. 2003; 33(5): 461-471.
- Avgustinovich D.F., Gorbach O.V., Kudryavtseva N.N. Comparative analysis of anxiety-like behavior in partition and plus-maze tests after agonistic interactions in mice. Physiol Behav. 1997; 61(1): 37-43.
- Kudryavtseva N.N., Avgustinovich D.F. Behavioral and physiological markers of experimental depression induced by social conflicts (DISC). Aggress Behav. 1998; 24: 271-286.
- Kudryavtseva N.N. The sensory contact model for the study of aggressive and submissive behaviors in male mice. Aggress Behav. 1991; 17(5): 285-291.
- Rodgers R.J., Cole J.C. The elevated plusmaze: pharmacology, methodology and ethology. In: Cooper S.J., Hendrie C.A. (eds). Ethology and Psychopharmacology. Chichester: John Wiley & Sons Ltd; 1994: 9-44.
- File S.E., Hyde J.R.G. Can social interaction be used to measure anxiety? Brit J Pharmacol. 1978; 62: 19-24.
- Kudryavtseva N.N., Bondar N.P., Avgustinovich D.F. Association between repeated experience of aggression and anxiety in male mice. Behav Brain Res. 2002; 133(1): 83-93.
- Denmark A., Tien D., Wong K., Chung A., Cachat J., Goodspeed J., Grimes C., Elegante M., Suciu C., Elkhayat S., Bartels B., Jackson A., Rosenberg M., Chung K.M., Badani H., Kadri F., Roy S., Tan J., Gaikwad S., Stewart A., Zapolsky I., Gilder T., Kalueff A.V. The effects of chronic social defeat stress on mouse self-grooming behavior and its patterning. Behav Brain Res. 2010; 208(2): 553-559.
- Липина Т.В., Кудрявцева Н.Н. Изменение исследовательского поведения у самцов мышей линии СВА/Lac под влиянием позитивного и негативного опыта социальных взаимодействий. Журн высш нервн деят им И.П. Павлова. 2008; 58(2): 194-201.
- Gillott A., Furniss F., Walter A. Anxiety in highfunctioning children with autism. Autism. 2001; 5(3): 277-286.
- White S.W., Oswald D., Ollendick T., Scahill L. Anxiety in children and adolescents with autism spectrum disorders. Clin Psychol Rev. 2009; 29(3): 216-229.
- Filipenko M.L., Beilina A.G., Alekseyenko O.V., Dolgov V.V., Kudryavtseva N.N. Repeated experience of social defeats increases serotonin transporter and monoamine oxidase A mRNA levels in raphe nuclei of male mice. Neurosci Lett. 2002; 321(1-2): 25-28.
- Berton O., McClung C.A., Dileone R.J., Krishnan V., Renthal, W., Russo S.J., Graham D., Tsankova N.M., Bolanos C.A., Rios M., Monteggia L.M., Self D.W., Nestler E.J., Essential role of BDNF in the mesolimbic dopamine pathway in social defeat stress. Science. 2006; 311: 864-868.
- Boer U., Alejel T., Beimesche S., Cierny I., Krause D., Knepel W., Flugge G., CRE/CREB-driven up-regulation of gene expression by chronic social stress in CRE-luciferase transgenic mice: reversal by antidepressant treatment. PLoS One. 2007; 2: e431.
- Pizarro J.M., Lumley L.A., Medina W., Robison C.L., Chang W.E., Alagappan A., Bah M.J., Dawood M.Y., Shah J.D., Mark B., Kendall N., Smith M.A., Saviolakis G.A., Meyerhoff J.L. Acute social defeat reduces neurotrophin expression in brain cortical and subcortical areas in mice. Brain Res. 2004; 1025: 10-20.
- Августинович Д.Ф., Коваленко И.Л., Кудрявцева Н.Н. Модель тревожной депрессии: персистентность патологии поведения. Росс физиол журн им И.М. Сеченова. 2004; 90(10): 1235-1244.
- Bondar N.P., Boyarskikh U.A., Kovalenko I.L., Filipenko M.L., Kudryavtseva N.N. Molecular implications of repeated aggression: Th, Dat1, Snca and Bdnf gene expression in the VTA of victorious male mice. PLoS One. 2009; 4(1): e4190.
- Beaudet A.L. Autism: highly heritable but not inherited. Nat Med. 2007; 13(5): 534-536.
- Freitag C.M. The genetics of autistic disorders and its clinical relevance: a review of the literature. Mol Psychiatry. 2007; 12(1): 2-22.
- Arndt T.L., Stodgell C.J., Rodier P.M. The teratology of autism. Int J Dev Neurosci. 2005; 23(2-3): 189-199.
- Kolevzon A., Gross R., Reichenberg A. Prenatal and perinatal risk factors for autism. Arch Pediatr Adolesc Med. 2007; 161(4): 326-33.
- Kinney D.K., Munir K.M., Crowley D.J., Miller A.M. Prenatal stress and risk for autism. Neurosci Biobehav Rev. 2008; 32(8): 1519-32.
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