The influence of glyprolins on the behavior of laboratory animals under experimental hyperthyroidism

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Abstract

This study aimed to determine the peculiarities of behavioral reactions and psychoemotional status of laboratory animals in experimental hyperthyroidism conditions against the background of introducing peptides of glyproline series (selank and tripeptide Pro-Gly-Pro). All experiments were conducted on white non-linear male rats, which were divided into groups consisting of 10 individuals. The first group consisted of control rats that received intraperitoneal water for injection in equi-volume. The second group consisted of individuals with experimental hyperthyroidism modeled by intragastric administration of L-thyroxine “Berlin-Chemi” Germany) at 150 mcg/kg/day for 21 days. The third and fourth groups were animals with a hyperthyroidism model and received intraperitoneal Selank and Pro-Gly-Pro at 200 mcg/kg/day for 21 days after the hyperthyroidism model development. The behavioral activity determination of laboratory animals was conducted using the “Open Field” method, which is widely used in the analysis of approximate research activity, locomotive stereotyping, and the emotional reactivity level of rodents. The level of animal anxiety was assessed using the Raised cross-shaped maze method. The study revealed that experimental hyperthyroidism due to the introduction of L-thyroxine is accompanied by an increased level of the anxiety-depressive state in the behavior of male rats. The study on the effects of selank and its structural analog Pro-Gly-Pro on the psychoemotional status of male rats using Open Field and Raised cruciform labyrinth methods revealed that the recovery of the approximate research motor activity of laboratory animals is noted, which indicates the psychomodulatory activity of glyproline neuropeptides, in experimental hyperthyroidism conditions.

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About the authors

Mariyam U. Sergalieva

Astrakhan State Medical University

Author for correspondence.
Email: charlina_astr@mail.ru
ORCID iD: 0000-0002-9630-2913
SPIN-code: 7976-9321

candidate of biological sciences

Russian Federation, Astrakhan

Alexsandra A. Tsibizova

Astrakhan State Medical University

Email: sasha3633@yandex.ru
ORCID iD: 0000-0002-9994-4751
SPIN-code: 2206-3898

candidate of pharmaceutical sciences

Russian Federation, Astrakhan

Elvira I. Abdulkadyrova

Astrakhan State Medical University

Email: elvira_abdulkadyrova@mail.ru

graduate student

Russian Federation, Astrakhan

Ludmila A. Andreeva

Kurchatov Institute — Institute of Molecular Genetics

Email: landr@img.ras.ru
ORCID iD: 0000-0002-3927-8590
SPIN-code: 4785-5621

sector manager

Russian Federation, Moscow

Marina A. Samotrueva

Astrakhan State Medical University

Email: ms1506@mail.ru
ORCID iD: 0000-0001-5336-4455
SPIN-code: 5918-1341

doctor of medical sciences, professor

Russian Federation, Astrakhan

Nikolay F. Myasoedov

Kurchatov Institute — Institute of Molecular Genetics

Email: nfm@img.ras.ru
ORCID iD: 0000-0003-1294-102X
SPIN-code: 1262-2698

doctor of chemical sciences, professor

Russian Federation, Moscow

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2. Fig. 1. The effects of Selang and Pro-Gly-Pro on motor activity of male rats under experimental hyperthyroidism in the "Open Field" method Hereinafter: * — p < 0.05; ** — p < 0.01; *** — p < 0.001 compared with controls; # — p < 0.05; ## — p < 0.01; ### — p < 0.001 compared with animal models of hyperthyroidism

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3. Fig. 2. The effect of Selang and Pro-Gly-Pro on the anxiety level of male rats under experimental hyperthyroidism in the "Open Field" method

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4. Fig. 3. The effects of Selang and Pro-Gly-Pro on the motor activity of male rats under experimental hyperthyroidism in the "Raised cross-shaped maze" method

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5. Fig. 4. The effects of Selang and Pro-Gly-Pro on the estimated research activity of male rats under experimental hyperthyroidism in the "Raised cross-shaped maze" method

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6. Fig. 5. The effects of Selang and Pro-Gly-Pro on the research activity of male rats under experimental hyperthyroidism in the "Raised cross-shaped maze" method Количество поведенческих, % актов

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7. Fig. 6. The effects of Selang and Pro-Gly-Pro on the anxiety of male rats under experimental hyperthyroidism in the "Raised cross-shaped maze" method

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Copyright (c) 2021 Sergalieva M.U., Tsibizova A.A., Abdulkadyrova E.I., Andreeva L.A., Samotrueva M.A., Myasoedov N.F.

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