Effect of mesenchymal stem cell transplantation on the structural and functional parameters of the liver in acute toxic hepatitis in rats

封面


如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Acute toxic hepatitis is a serious health problem for the working population. The currently widely used pharmacological preparations with claimed hepatoprotective properties for the treatment of liver diseases have dubious therapeutic efficacy and cannot restore functional and structural changes in the damaged organ. Thus, the search for new methods of treating liver diseases, including toxic etiology, is an urgent task. An experimental study was conducted on 134 nonlinear white outbred rats (3–5 months) weighing 250–390 g. The animals were divided into four groups: group I, control group (n = 15); group II, single intragastric administration of an oil solution of carbon tetrachloride at a dose of 1500 mg/kg (n = 41); group III, single intravenous administration of multipotent mesenchymal stem cells of the mouse (n = 20); group IV, single intragastric administration of an oil solution of carbon tetrachloride at a dose of 1500 mg/kg and subsequent intravenous transplantation of multipotent mesenchymal stem cells of the mouse (2 × 106) (n = 58). On days 1, 3, 5, and 7 of the experiment, blood sampling was performed for biochemical analysis, and organs and tissues were selected for histological and morphometric studies. The transplantation of multipotent mouse mesenchymal stem cells reduced the mortality of animals after exposure to carbon tetrachloride and did not cause acute toxicity. According to a morphometric study, starting from day 3, the introduction of multipotent mouse mesenchymal stem cells led to a statistically significant decrease in the infiltrative processes in the liver tissue. Statistically significant changes in the biochemical composition of the rat blood serum were noted against the transplantation of multipotent mouse mesenchymal stem cells: a decrease in the activities of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, gamma-glutamyltransferase, amylase, creatinine, uric acid, direct and total bilirubin, and cholesterol. The transplantation of multipotent mouse mesenchymal stem cells was found to be an effective method of treating acute toxic hepatitis. The use of multipotent mouse mesenchymal stem cells contributed to an increase in the survival rate of laboratory animals and faster recovery of structural and functional disorders in liver tissue after its acute toxic damage.

全文:

受限制的访问

作者简介

Elena Trapeznikova

North-Western State Medical University named after I.I. Mechnikov

编辑信件的主要联系方式.
Email: pishite22@mail.ru
ORCID iD: 0000-0002-9334-4042
SPIN 代码: 8588-4013

graduate student

俄罗斯联邦, Saint Petersburg

Viktor Shilov

North-Western State Medical University named after I.I. Mechnikov

Email: pishite22@mail.ru
ORCID iD: 0000-0003-3256-2609
SPIN 代码: 3541-4782
Scopus 作者 ID: 7102157390

doctor of medical sciences, professor

俄罗斯联邦, Saint Petersburg

参考

  1. Lo Re V III, Haynes K, Forde KA, et al. Risk of acute liver failure in patients with drug-induced liver injury: evaluation of Hy’s Law and a new prognostic model. Clin Gastroenterol Hepatol. 2015;13(13):2360–2368. doi: 10.1016/j.cgh.2015.06.020
  2. Teschke R. Alcoholic steatohepatitis (ASH) and alcoholic hepatitis (AH): Cascade of events, clinical aspects, and pharmacotherapy options. Expert Opin Pharmacother. 2018;19(8):779–793. doi: 10.1080/14656566.2018.1465929
  3. Licata A. Adverse drug reactions and organ damage: The liver. Eur J Intern Med. 2016;28:9–16. doi: 10.1016/j.ejim.2015.12.017
  4. sostoyanii sanitarno-ehpidemiologicheskogo blagopoluchiya naseleniya v Rossiiskoi Federatsii v 2021 godu: Gosudarstvennyi doklad. Federal’naya sluzhba po nadzoru v sfere zashchity prav potrebitelei i blagopoluchiya cheloveka. Moscow: Federal’naya sluzhba po nadzoru v sfere zashchity prav potrebitelei i blagopoluchiya cheloveka; 2022. 340 p. (In Russ.).
  5. Kirzhanova VV, Grigorova NI, Bobkov EN, et al. Sostoyanie i deyatel’nost’ narkologicheskoi sluzhby v Rossiiskoi Federatsii v 2021 godu. Moscow: NMITS PN im. V.P. Serbskogo; 2022. 202 p. (In Russ.).
  6. Kudasheva AR, Teregulova ZS, Khusainova AKh, et al. Professional’nye zabolevaniya gepatobiliarnoi sistemy: uchebnoe posobie. Ufa: BGMU, 2018. 97 p. (In Russ.).
  7. Marjani M, Fahim F, Sadr M, et al. Evaluation of Silymarin for Management of Anti-tuberculosis Drug Induced Liver Injury: a Randomized Clinical Trial. Gastroenterol Hepatol Bed Bench. 2019;12(2):138–142.
  8. Niu H, Sanabria-Cabrera J, Alvarez-Alvarez I, et al. Prevention and management of idiosyncratic drug-induced liver injury: Systematic review and meta-analysis of randomised clinical trials. Pharmacol Res. 2021;164:105404. doi: 10.1016/j.phrs.2020.105404
  9. Niu H, Atallah E, Alvarez-Alvarez I, et al. Therapeutic Management of Idiosyncratic Drug-Induced Liver Injury and Acetaminophen Hepatotoxicity in the Paediatric Population: A Systematic Review. Drug Saf. 2022;45(11):1329–1348. doi: 10.1007/s40264-022-01224-w
  10. Wang Y, Wang Z, Gao M, et al. Efficacy and Safety of Magnesium Isoglycyrrhizinate Injection in Patients with Acute Drug-Induced Liver Injury: A Phase II Trial. Liver Int. 2019;39(11):2102–2111. doi: 10.1111/liv.14204
  11. Yagi H, Soto-Gutierrez A, Parekkadan B, et al. Mesenchymal stem cells: Mechanisms of immunomodulation and homing. Cell Transplant. 2010;19(6):667–679. doi: 10.3727/096368910X508762
  12. Naderi-Meshkin H, Bahrami AR, Bidkhori HR, et al. Strategies to improve homing of mesenchymal stem cells for greater efficacy in stem cell therapy. Cell Biol Int. 2015;39(1):23–34. doi: 10.1002/cbin.10378
  13. Diomede F, Gugliandolo A, Cardelli P, et al. Three-dimensional printed PLA scaffold and human gingival stem cell-derived extracellular vesicles: a new tool for bone defect repair. Stem Cell Res Ther. 2018;9:104. doi: 10.1186/s13287-018-0850-0
  14. Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. the international society for cellular therapy position statement. Cytotherapy. 2006;8(4):315–317. doi: 10.1080/14653240600855905
  15. Tasso R, Ilengo C, Quarto R, et al. Mesenchymal stem cells induce functionally active T-regulatory lymphocytes in a paracrine fashion and ameliorate experimental autoimmune uveitis. Investig Ophthalmol Vis Sci. 2012;53(2):786–793. doi: 10.1167/iovs.11-8211
  16. Liu M, Zeng X, Wang J, et al. Immunomodulation by mesenchymal stem cells in treating human autoimmune disease-associated lung fibrosis. Stem Cell Res Ther. 2016;7:63. doi: 10.1186/s13287-016-0319-y
  17. Budoni M, Fierabracci A, Luciano R, et al. The immunosuppressive effect of mesenchymal stromal cells on B lymphocytes is mediated by membrane vesicles. Cell Transplant. 2013;22(2):369–379. doi: 10.3727/096368911X582769b
  18. Squillaro T, Peluso G, Galderisi U. Clinical Trials With Mesenchymal Stem Cells: An Update. Cell Transplant. 2016;25(5):829–848. doi: 10.3727/096368915X689622
  19. Naji A, Eitoku M, Favier B, et al. Biological functions of mesenchymal stem cells and clinical implications. Cell Mol Life Sci. 2019;76(17):3323–3348. doi: 10.1007/s00018-019-03125-1
  20. Liu Y, Dong Y, Wu X, et al. The assessment of mesenchymal stem cells therapy in acute on chronic liver failure and chronic liver disease: a systematic review and meta-analysis of randomized controlled clinical trials. Stem Cell Res Ther. 2022;13(1):204. doi: 10.1186/s13287-022-02882-4
  21. Benavides-Castellanos MP, Garzón-Orjuela N, Linero I. Effectiveness of mesenchymal stem cell-conditioned medium in bone regeneration in animal and human models: a systematic review and meta-analysis. Cell Regen. 2020;9(1):5. doi: 10.1186/s13619-020-00047-3
  22. Karytka AA, Krivenko SI, Shcherba AE, et al. Role of mesenchymal stem cells in maintaining the viability and functional activity of hepatocyte culture in vitro. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2017;(1):7–14. (In Russ.).
  23. Mohamadnejad M, Alimoghaddam K, Bagheri M, et al. Randomized placebo-controlled trial of mesenchymal stem cell transplantation in decompensated cirrhosis. Liver Int. 2013;33(10):1490–1496. doi: 10.1111/liv.12228
  24. Rehab AM, Heba MS, Laila AR, et al. Combined effect of hydrogen sulfide and mesenchymal stem cells on mitigating liver fibrosis induced by bile duct ligation: Role of anti-inflammatory, anti-oxidant, anti-apoptotic, and anti-fibrotic biomarkers. Iran J Basic Med Sci. 2021;24(12):1753–1762. doi: 10.22038/IJBMS.2021.56477.12604
  25. Zhang L, Zhou D, Li J, et al. Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Hypoxia and the Transforming Growth Factor beta 1 (TGFβ-1) and SMADs Pathway in a Mouse Model of Cirrhosis. Med Sci Monit. 2019;25:7182–7190. doi: 10.12659/MSM.916428
  26. Popp FC, Renner P, Eggenhofer E, et al. Mesenchymal stem cells as immunomodulators after liver transplantation. Liver Transpl. 2009;15(10):1192–1198. doi: 10.1002/lt.21862
  27. Joo S-Y, Cho K-A, Jung Y-J, et al. Mesenchymal stromal cells inhibit graft-versus-host disease of mice in a dose-dependent manner. Cytotherapy. 2010;12(3):361–370. doi: 10.3109/1465
  28. Li ZY, Wang CQ, Lu G, et al. Effects of bone marrow mesenchymal stem cells on hematopoietic recovery and acute graft-versus-host disease in murine allogeneic umbilical cord blood transplantation model. Cell Biochem Biophys. 2014;70(1):115–122. doi: 10.1007/s12013-014-9866-y
  29. Budgude P, Kale V, Vaidya A. Mesenchymal stromal cell-derived extracellular vesicles as cell-free biologics for the ex vivo expansion of hematopoietic stem cells. Cell Biol Int. 2020;44(5):1078–1102. doi: 10.1002/cbin.11313
  30. Trapeznikova ЕG, Popov VВ, Radilov AS, Shilov VV. Dose-dependent character of disturbance of hematopoietic and immune systems function, production of some hormones in experimental uranium acetate dihydrate exposure. Toxicological Review. 2021;(1):14–19. (In Russ.) doi: 10.36946/0869-7922-2021-1-14-19

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Eco-Vector, 2023


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 77762 от 10.02.2020.


##common.cookie##