Modulating effect of recombinant lactoferrin isolated from the milk of transgenic goats on platelet aggregation activity
- Authors: Shamova E.V.1, Grigorieva D.V.1, Gorudko I.V.1, Sveshnikova A.N.2,3
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Affiliations:
- Belarusian State University
- Lomonosov Moscow State University
- Center for Theoretical Problems of Physicochemical Pharmacology of Russian Academy of Sciences
- Issue: Vol 18, No 4 (2018)
- Pages: 58-63
- Section: Articles
- Published: 15.12.2018
- URL: https://journals.eco-vector.com/MAJ/article/view/11686
- DOI: https://doi.org/10.17816/MAJ18458-63
- ID: 11686
Cite item
Abstract
This work showed for the first time that recombinant lactoferrin isolated from the milk of transgenic goats binds to the plasma membrane of platelets. The interaction of recombinant lactoferrin with platelets leads to the potentiation or inhibition of agonist-induced platelet aggregation depending on the type of agonist (adenosine diphosphate, thrombin, plant lectins WGA, Con A, and SNA). Using fluorescently labeled antibody CD42b it was shown by flow cytometry that recombinant lactoferrin binds to GPIb on platelets.
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About the authors
Ekaterina V. Shamova
Belarusian State University
Author for correspondence.
Email: shamova@tut.by
PhD in Biology, Senior Research Scientist, Research Laboratory of Biophysics and Biotechnology, Department of Biophysics, Faculty of Physics
Belarus, 4, Nezavisimosti Ave., 220030, MinskDaria V. Grigorieva
Belarusian State University
Email: dargr@tut.by
PhD in Biology, Senior Research Scientist, Research Laboratory of Biophysics and Biotechnology, Department of Biophysics, Faculty of Physics
Belarus, 4, Nezavisimosti Ave., 220030, MinskIrina V. Gorudko
Belarusian State University
Email: irinagorudko@gmail.com
PhD in Biology, Associate Professor, Associate Professor of the Department of Biophysics, Faculty of Physics
Russian Federation, 4, Nezavisimosti Ave., 220030, MinskAnastasia N. Sveshnikova
Lomonosov Moscow State University; Center for Theoretical Problems of Physicochemical Pharmacology of Russian Academy of Sciences
Email: agolomy@gmail.com
PhD of Physics and Mathematics, Senior research scientist, Department of Biophysics, Faculty of Physics; Head of the Laboratory of Intracellular Signaling and Systems Biology
Russian Federation, 1, Leninskie gory, Moscow, 119991; 4, Kosigina street, Moscow, 119991References
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