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Original experimental model of tuberculosis and lung cancer
- Authors: Kudriashov G.G.1, Nefedov A.O.1, Tochilnikov G.V.2, Zmitrichenko Y.G.2, Krylova Y.S.1, Dogonadze M.Z.1, Zabolotnyh N.V.1, Dyakova M.E.1, Esmerdyaeva D.S.1, Vitovskaya M.L.1, Gavrilov P.V.1, Azarov A.A.1, Zhuravlev V.Y.1, Vinogradova T.I.1, Yablonskii P.K.1
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Affiliations:
- St. Petersburg Research Institute of Phthisiopulmonology
- N.N. Petrov Oncology Research Institute
- Issue: Vol 13, No 5 (2022)
- Pages: 33-42
- Section: Original studies
- URL: https://journals.eco-vector.com/pediatr/article/view/110977
- DOI: https://doi.org/10.17816/PED13533-42
- ID: 110977
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Abstract
BACKGROUND: The potential relationship between pulmonary tuberculosis and lung cancer has been the subject of intense interest over the past few decades. Nevertheless, the features of the pathogenesis of concomitant pathology remain poorly studied.
AIM: The ain of the study to develop an experimental model of the concomitant pathology of tuberculosis and lung cancer.
MATERIALS AND METHODS: The study was performed on mice of the C57BL/6 line at the age of two months in four groups: 1st — intact mice (n = 12), 2nd — mice without a tumor infected with tuberculosis (n = 24), 3rd — tumor-bearing mice not infected with tuberculosis (n = 23), 4th — tumor-bearing mice infected with tuberculosis (n = 24). Individual and group parameters were evaluated using the SPSS Statistica v23 software package.
RESULTS: Tumor was developed at the site of primary transplantation in all mice from groups 3 and 4, which was confirmed by visual assessment and the results of histological examination. Tumor growth in the main group was significantly less than in the control group of the tumor, which may be due to intoxication against the background of tuberculosis infection. All infected mice from groups 2 and 4 developed pulmonary tuberculosis, confirmed by computed tomography of the lungs, bacteriological and histological examination of lung samples. Mycobacterial load in the lungs was the highest in animals with concomitant pathology of tuberculosis and tumor. The survival rate of mice was determined to a large extent by tumor growth rather than by the progression of tuberculosis infection.
CONCLUSIONS: The results of the study indicate the possibility of creating an experimental biological model of the concomitant pathology of tuberculosis and lung cancer in mice. The features of the course of the concomitant pathology were revealed: Lewis lung epidermoid carcinoma develops more slowly in tuberculosis infected animals than in the tumor control group; the development of the tuberculosis process in mice with a tumor occurs more intensively than in the tuberculosis infection control group. The survival rate of mice with concomitant pathology is determined more by the intensity of tumor growth than by the progression of tuberculosis.
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About the authors
Grigorii G. Kudriashov
St. Petersburg Research Institute of Phthisiopulmonology
Author for correspondence.
Email: dr.kudriashov.gg@yandex.com
MD, PhD, Senior Research Associate, Thoracic Surgery Center
Russian Federation, Saint PetersburgAndrey O. Nefedov
St. Petersburg Research Institute of Phthisiopulmonology
Email: herurg78@mail.ru
MD, PhD, Senior Research Associate, Thoracic Surgery Center
Russian Federation, Saint PetersburgGrigorii V. Tochilnikov
N.N. Petrov Oncology Research Institute
Email: gr75@mail.ru
MD, PhD, Head of the Scientific Laboratory of Cancer Chemoprophylaxis and Oncopharmacology
Russian Federation, Saint PetersburgYuliya G. Zmitrichenko
N.N. Petrov Oncology Research Institute
Email: zmitrichenko@gmail.com
Junior Research Associate, Scientific Laboratory of Cancer Chemoprophylaxis and Oncopharmacology
Russian Federation, Saint PetersburgYuliya S. Krylova
St. Petersburg Research Institute of Phthisiopulmonology
Email: emerald2008@mail.ru
MD, PhD, Senior Research Associate, Center for Molecular Biomedicine
Russian Federation, Saint PetersburgMarine Z. Dogonadze
St. Petersburg Research Institute of Phthisiopulmonology
Email: marine-md@mail.ru
MD, PhD, Senior Research Associate
Russian Federation, Saint PetersburgNataliya V. Zabolotnyh
St. Petersburg Research Institute of Phthisiopulmonology
Email: info@spbniif.ru
MD, PhD, Dr. Sci. (Med.), Leading Research Associate
Russian Federation, Saint PetersburgMarina E. Dyakova
St. Petersburg Research Institute of Phthisiopulmonology
Email: info@spbniif.ru
MD, PhD, Senior Research Associate
Russian Federation, Saint PetersburgDilyara S. Esmerdyaeva
St. Petersburg Research Institute of Phthisiopulmonology
Email: info@spbniif.ru
MD, PhD, Senior Research Associate
Russian Federation, Saint PetersburgMaria L. Vitovskaya
St. Petersburg Research Institute of Phthisiopulmonology
Email: mariavit72@mail.ru
MD, PhD, Senior Research Associate
Russian Federation, Saint PetersburgPavel V. Gavrilov
St. Petersburg Research Institute of Phthisiopulmonology
Email: spbniifrentgen@mail.ru
MD, PhD, Leading Research Associate
Russian Federation, Saint PetersburgArtem A. Azarov
St. Petersburg Research Institute of Phthisiopulmonology
Email: azardoc0@gmail.com
Postgraduate Student
Russian Federation, Saint PetersburgVyacheslav Yu. Zhuravlev
St. Petersburg Research Institute of Phthisiopulmonology
Email: vy.zhuravlev@spbniif.ru
MD, PhD, Leading Research Associate
Russian Federation, Saint PetersburgTatyana I. Vinogradova
St. Petersburg Research Institute of Phthisiopulmonology
Email: ti.vinogradova@spbniif.ru
MD, PhD, Dr. Sci. (Med.), Project Leader
Russian Federation, Saint PetersburgPiotr K. Yablonskii
St. Petersburg Research Institute of Phthisiopulmonology
Email: piotr_yablonskii@mail.ru
MD, PhD, Dr. Sci. (Med.), Director
Russian Federation, Saint PetersburgReferences
- Vasil’eva EB, Klochkova LV, Korol’ OI, et al. Tuberkulez u detei i podrostkov. Saint Petersburg: Piter, 2005. (In Russ.)
- Vershinina SF, Stukov AN. Ehksperimental’nye opukholi: Prakticheskoe rukovodstvo. Saint Petersburg: Galanika, 2018. 68 p. (In Russ.)
- Gavrilov PV, Vinogradova TI, Azarov AA, et al. Radiation and functional diagnostics. Medical Alliance. 2020;(4):65–72. (In Russ.) doi: 10.36422/23076348-2020-8-4-65-72
- Nikolaev AV, Utekhin VJ, Churilov LP. Comparative aetio-epidemiological analysis of lung tuberculosis versus sarcoidosis: classical and new concepts. Pediatrician (St. Petersburg). 2020;11(5):37–50. (In Russ.) doi: 10.17816/PED11537-50
- Pavlova MV, Ershova ES, Vinogradova TI, et al. Modern trends in treatment of drug-resistant tuberculosis. Medical Alliance. 2017;(4):23–29. (In Russ.)
- Yablonskiy PK, Vinogradova TI, Pavlova MV, et al. Novye perspektivy lecheniya tuberkuleza organov dykhaniya. Medical Alliance. 2013;(2):69–73. (In Russ.)
- Yablonskiy PK, Avetisyan AO, Chausov AV, et al. The case of successful surgical treatment of a combination of central squamous cell lung cancer with invasiveinto the atrium and active destructive pulmonary tuberculosis with the use of an cardiopulmonary bypass. Medical Alliance. 2018;(2):77–84. (In Russ.)
- Engels EA, Shen M, Chapman RS, et al. Tuberculosis and subsequent risk of lung cancer in Xuanwei, China. Int J Cancer. 2009;124(5):1183–1187. doi: 10.1002/ijc.24042
- Heuvers ME, Aerts JG, Hegmans JPJV, et al. History of tuberculosis as an independent prognostic factor for lung cancer survival. Lung Cancer. 2012;76(3): 452–456. doi: 10.1016/j.lungcan.2011.12.008
- Kuo C-H, Lo C-Y, Chung F-T, et al. Concomitant active tuberculosis prolongs survival in non-small cell lung cancer: a study in a tuberculosis-endemic country. PLoS One. 2012;7(3): e33226. doi: 10.1371/journal.pone.0033226
- Yu Y-H, Liao C–C, Hsu W-H, et al. Increased lung cancer risk among patients with pulmonary tuberculosis: a population cohort study. J Thorac Oncol. 2011;6(1):32–37. doi: 10.1097/JTO.0b013e3181fb4fcc
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