No inducible synthase inhibitors produce a positive effect on indexes of central hemodynamics in rats with septic shock

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Abstract

The purpose of the study was to evaluate the efficacy of NO inducible synthase (iNOS) inhibitors involved in simulation of septic shock in rats. Model of septic shock (SS) was obtained in rats by intravenous administration of live Bifidobacteria culture. Aminoguanidine was used as a selective inhibitor of iNOS. Indexes of central hemodynamics measured by direct method where analyzed. Vascular endothelial function was rated by determination of nitric oxide (II) (NO), vascular endothelial growth factor A (VEGF-A) and tissue plasminogen activator (tPA) in plasma. Hemostatic parameters were evaluated by the level of soluble fibrin monomer complexes (RKMF). Intravenous administration of live Bifidobacteria culture in rats proved to cause an adequate and effective septic shock model with characteristic changes of hemodynamics in laboratory animals, development of endothelial dysfunction and hemostasis system activation in response to bacteremia. INOS inhibitors have a positive impact on hemodynamics in septic shock. NO is not the only factor in the development of hypotension in septic shock. INOS inhibitors appear to affect the extent of vasopressor dose utilized in the therapy of septic shock contributing to improvement of microcirculation and tissue oxygenation.

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About the authors

Danila Viktorovich Strukov

St. Petersburg State Pediatric Medical University

Email: dstrukov@yandex.ru
Postgraduate Student

Andrey Glebovich Vasiliev

St. Petersburg State Pediatric Medical University

Email: avas7@mail.ru
MD, PhD, Dr Med Sci, Head, Dept. of Pathophysiology

Yuriy Stanislavovich Alexandrovich

St. Petersburg State Pediatric Medical University

Email: jalex1963@mail.ru
MD, PhD, Dr Med Sci, Head, Dept. of Aneasthesiolgic and reanimatologic

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Copyright (c) 2015 Strukov D.V., Vasiliev A.G., Alexandrovich Y.S.

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