Study of the specific activity of an innovative dosage form of a chemotherapeutic agent
- Authors: Naryshkin S.R.1, Krasnyuk I.I.1, Krasnyuk I.I.1, Fateeva T.V.2, Mizina P.G.2, Belyatskaya A.V.1, Stepanova O.I.1, Smolyarchuk E.A.1, Plakhotnaia O.N.1, Bokov D.O.1, Grikh V.V.1, Boldina M.S.1, Rastopchina O.V.1, Bobrov I.S.1
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Affiliations:
- Sechenov First Moscow State Medical University (Sechenov University)
- All-Russian research Institute of Medicinal and Aromatic plants
- Issue: Vol 71, No 4 (2022)
- Pages: 19-25
- Section: Pharmaceutical chemistry and pharmacognosy
- URL: https://journals.eco-vector.com/0367-3014/article/view/113735
- DOI: https://doi.org/10.29296/25419218-2022-04-03
- ID: 113735
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Abstract
Introduction. On the basis of Sechenov University departments, an innovative antimicrobial and antiprotozoal drug – metronidazole in the form of effervescent tablets has been developed. Together with the «All-Russian research Institute of Medicinal and Aromatic plants», the antimicrobial activity of the developed instant tablets was studied. Metronidazole has been successfully used for more than 60 years to treat diseases of the gastrointestinal tract, genitourinary system; it is actively used in dental and dermatological practice. At the same time, there are currently no instant dosage forms of metronidazole on the pharmaceutical market of the Russian Federation that allow patients to use the drug for both internal and topical use.
Objective: to determine the antimicrobial activity of an experimentally developed new dosage form of metronidazole – effervescent tablets in comparison with ready-made dosage forms of metronidazole: infusion solution and tablets.
Material and methods. Metronidazole (substance), developed effervescent metronidazole tablets (composition No. 2 and No. 3), their placebo, sodium benzoate (auxiliary substance – sliding), industrially manufactured tablets and infusion solution. Determination of bacteriostatic and fungistatic activity was studied by dilution method. Pathogenic gram-positive and gram-negative bacteria, yeast-like and mycelial fungi were used. When determining the bacteriostatic activity of the studied samples, meat-peptone broth was used. When determining the fungistatic activity – a liquid Saburo medium.
Results. The data obtained indicate that all samples containing metronidazole in their composition practically coincide in antimicrobial (bacteriostatic and fungistatic) activity with each other and with the activity of the metronidazole substance included in their composition, which indirectly confirms the complete release of the substance from experimental dosage forms and the absence of formation of insoluble complexes with placebo that could inhibit the release of metronidazole.
Conclusion. The conducted studies have established the presence of antimicrobial activity in the developed effervescent metronidazole tablets.
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About the authors
Savva Ruslanovich Naryshkin
Sechenov First Moscow State Medical University (Sechenov University)
Email: savva.naryshkin@gmail.com
ORCID iD: 0000-0003-1775-4805
postgraduate student, department of analytical, physical and colloid chemistry, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Ivan Ivanovich Krasnyuk
Sechenov First Moscow State Medical University (Sechenov University)
Email: krasnyuk.79@mail.ru
ORCID iD: 0000-0001-8557-8829
professor, doctor of pharmaceutical science, PhD, head of department of analytical, physical and colloid chemistry, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Ivan Ivanovich Krasnyuk
Sechenov First Moscow State Medical University (Sechenov University)
Email: krasnyuki@mail.ru
ORCID iD: 0000-0002-7242-2988
professor, doctor of pharmaceutical science, head of department of pharmaceutical technology, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Tatiana Vladimirovna Fateeva
All-Russian research Institute of Medicinal and Aromatic plants
Email: fateeva2151@mail.ru
ORCID iD: 0000-0002-8231-0621
head of the microbiological research laboratory, leading researcher
Russian Federation, st. Grina, 7/1, Moscow, 117216Prascovia Georgievna Mizina
All-Russian research Institute of Medicinal and Aromatic plants
Email: pg-mizina@vilarnii.ru
deputy director for scientific work, professor, doctor of pharmaceutical science
Russian Federation, st. Grina, 7/1, Moscow, 117216Anastasiya Vladimirovna Belyatskaya
Sechenov First Moscow State Medical University (Sechenov University)
Email: av.beliatskaya@mail.ru
ORCID iD: 0000-0002-8214-4483
Candidate of pharmaceutical sciences, docent of department of pharmaceutical technology, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Ol'ga Ivanovna Stepanova
Sechenov First Moscow State Medical University (Sechenov University)
Email: o.i.nikulina@mail.ru
ORCID iD: 0000-0002-9885-3727
candidate of pharmaceutical science, docent of department of pharmacology, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Elena Anatol'evna Smolyarchuk
Sechenov First Moscow State Medical University (Sechenov University)
Email: smolyarchuk@mail.ru
ORCID iD: 0000-0002-2615-7167
candidate of medical science, docent, head of department of pharmacology, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Ol'ga Nikolaevna Plakhotnaia
Sechenov First Moscow State Medical University (Sechenov University)
Email: plahotnaya.o@mail.ru
ORCID iD: 0000-0001-7266-2933
candidate of chemical science, docent of department of analytical, physical and colloid chemistry, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Dmitrij Olegovich Bokov
Sechenov First Moscow State Medical University (Sechenov University)
Email: fmmsu@mail.ru
ORCID iD: 0000-0003-2968-2466
candidate of pharmaceutical science, docent of department of analytical, physical and colloid chemistry, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Viktoriya Vladimirovna Grikh
Sechenov First Moscow State Medical University (Sechenov University)
Email: viktoriya.grikh@mail.ru
ORCID iD: 0000-0003-1614-1070
candidate of pharmaceutical science, docent of department of analytical, physical and colloid chemistry, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Maria Sergeevna Boldina
Sechenov First Moscow State Medical University (Sechenov University)
Email: mar-usy@yandex.ru
ORCID iD: 0000-0002-1620-699X
postgraduate student, department of analytical, physical and colloid chemistry, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Olga Vyacheslavovna Rastopchina
Sechenov First Moscow State Medical University (Sechenov University)
Email: rastop0309@yandex.ru
ORCID iD: 0000-0002-5443-6980
Candidate of pharmaceutical sciences, docent of department of pharmaceutical technology, Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991Ivan Sergeevich Bobrov
Sechenov First Moscow State Medical University (Sechenov University)
Author for correspondence.
Email: bobrov2602@yandex.ru
ORCID iD: 0000-0001-8263-5541
student of Institute of Pharmacy named after A.P. Nelyubin
Russian Federation, st. Trubetskaya, 8–2, Mosсow, 119991References
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