Regulation of the antioxidant defense system in the liver of white rats against the background of drug-induced hepatitis

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Abstract

Introduction. Medicinal hepatitis is one of the pathologies characteristic of modern times. The cause of the disease is taking medications without medical supervision and, on the other hand, an increase in the range of drugs with a hepatotoxic effect.

Purpose of the study. To study the antioxidant effect created at the Scientific Research Center of the Azerbaijan Medical University by Professor Kh.I. Gasanov of the complex compound (C8H12ON)2 [PdCl4], synthesized on the basis of palladium and mexidol.

Material and methods. To study its effect on a living organism, an experiment was conducted on 30 outbred white rats. The experimental animals were divided into 6 groups of 5 animals each.

Results and discussions. 3 days after the modeling of medicinal hepatitis in the liver tissue, a weakening of the antioxidant defense system was observed. The obtained results show that the model of medicinal hepatitis created using paracetamol was stable, and the concentration of markers of the antioxidant defense system in the homogenate continued to decrease, despite the 10th day after the creation of the pathological process. As a result of exposure to KSPM introduced into the body, the average concentration of markers of the antioxidant defense system in the liver homogenate increased significantly. There was also an increase in the concentration of PB-SH, the concentration of the internal group of the protein-SH, peroxidase activity, catalase activity and TAA.

Conclusion. It has been established that the drug increases the concentration of markers of the antioxidant defense system in the liver of white rats, created against the background of a hepatitis model. The effect of the synthesized biologically active substance depends on the time of its introduction into the body.

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About the authors

Ismail Mammad ogly Baghirov

Azerbaijan Medical University

Email: Mamed_2016@list.ru
ORCID iD: 0000-0003-0145-8390

Candidate of Philological Sciences, full-time doctoral student

Azerbaijan, 23, Bakikhanov str., Baku

Sevda Vagif kizi Guliyeva

Azerbaijan Medical University

Author for correspondence.
Email: sevda.quliyeva.1970@mail.ru
ORCID iD: 0000-0002-6064-4081

Candidate of Biological Sciences, Associate Professor, Head of the Department of Biochemistry of the Research Center

Azerbaijan, 23, Bakikhanov str., Baku

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2. Fig. 1. The proportion of white rats with a normal concentration of antioxidant markers in the liver homogenate in the 2nd and 4th groups. Note. 1–2 – surface and internal protein-SH groups; 3 – peroxidase; 4 – catalase; 5 – OAA.

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3. Fig. 2. The percentage of antioxidant markers in the liver of animals of the 3rd and 6th groups, which are above the norm

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