Vol 69, No 8 (2020)

Introduction. The perennial herbaceous plan Canadian goldenrod (Solidago canadensis) is a valuable source of flavonoids. The quality of Canadian goldenrod herb is assessed according to the pharmacopoeial article “Canadian goldenrod herb” designed more than 25 years ago, many sections of which require revision in accordance with modern requirements for the quality of plant raw materials. Objective: to develop and validate a quantitative measurement procedure for flavonoids in the Canadian goldenrod herb. Material and methods. The investigation objects were the Canadian goldenrod herb samples collected on the territory of the Leningrad Region and in the vicinity of Prague (Czech Republic) in the period of 2016 to 2018. Quantitative determination was carried out using differential spectrophotometry by the aluminum chloride (III)-flavonoid complexation reaction. The procedure was validated in terms of specificity, linearity, precision, repeatability, and robustness. Results. The investigators studied the impact of various technological parameters: the type of an extraction agent and its concentration; the time, frequency, and temperature of extraction; the degree of grinding of raw materials; the time of a reaction of flavonoids with a complexing agent (2% aluminum chloride solution), the quantity of a reagent for measuring the levels of flavonoids in the raw material. The optimal conditions for analysis were determined. Conclusion. A spectrophotometric quantitative measurement procedure for the total count of flavonoids calculated with reference to rutin in the Canadian goldenrod herb was developed and validated.

Introduction. The relevance of designing dosage forms for dental practice, including therapeutic and prophylactic toothpastes, is due to several factors. First of all, due to the fact that oral hygiene is one of the most important and most widespread preventive measures for oral infectious and inflammatory diseases. This in turn assists in preventing the diseases and conditions associated with chronic infection foci in the oral cavity. Therefore, hygienic care of the teeth and oral cavity not only prevents dental diseases, but also reduces the infectious load on the body as a whole. Objective: to develop a technology for therapeutic and prophylactic Dentos phytocomposition-based toothpaste, to investigate its characteristics and to work out some indicators of its quality, and to analyze the findings. Material and methods. The investigation objects were a Dentos herbal remedy based on five types of medicinal plant materials, as well as samples of various toothpaste compositions. The investigators used technological methods for preparing the toothpaste, analytical methods for assessing the quality indicators of the designed composition: methods for determining homogeneity, plasticity, pH value, the height of the foam column, and foam stability; and phytochemical methods for pharmacopoeial assays (qualitative test tube reactions, TLC, and UV spectrophotometry). Results. The authors provided evidence that it was expedient to design therapeutic and prophylactic herbal toothpastes made in Russia. The compositions of toothpastes based on sodium-CMC were obtained, by using the following abrasives: precipitated calcium carbonate, aerosil, and also Dentos tincture in various ratios. The composition that was optimal in terms of ensuring and preserving the organoleptic characteristics of the toothpaste was selected. The step-by-step technological scheme for the toothpaste was substantiated. The indicators characterizing its quality were identified. For a qualitative analysis, the investigators proposed to use the nature of the UV spectrum and a reaction with flavonoids and tannins. They also devised a method for the quantitative spectrophotometric determination of the amount of phenylpropanoids calculated with reference to caffeic acid. Conclusion. The composition of therapeutic and prophylactic toothpaste based on the Dentos herbal remedy was designed and its technology developed. The organoleptic and technological characteristics of the toothpaste were studied. The investigators proposed its quality indicators, such as appearance, color, odor, taste, mass fraction of water, hydrogen index (pH), foam height, foam stability, qualitative and quantitative determination of biologically active compounds.

Introduction. Different types of wounds can be acquired not only in a combat zone, but also in everyday life. In the absence of specialized medicines at hand, it is very important to primarily manage injuries. Preliminary data show that some gun oil samples have wound healing properties, which may be of practical use in the field. Objective: to investigate the wound healing effect of a sample of CUPPER gun oil on a rat skin wound model. Material and methods. The investigation object was a sample of CUPPER weapons oil based on high-molecular-weight ethers with a complex copper-containing lubricant antiwear additive. The positive control drug was Methyluracil® that belongs to a pharmaceutical group of tissue repair stimulant. Male outbred rats were used as a test system. A skin wound was simulated in the area of animals' withers. The course of the wound process was evaluated by clinical studies. A planimetric method was used to objectively assess wound healing. Results. Pronounced positive effects on the processes of tissue proliferation were observed when the oil sample was applied twice daily. An experiment revealed similar positive effects on the wound healing processes when applying Methyluracil® topically to the wound surface once daily and the test gun oil sample twice daily. The investigation results suggest that application of gun oil to the wound surface stimulates the regenerative processes. Conclusion. When topically applied, gun oil was found to have a wound healing effect on a male rat skin wound model, as confirmed by the results of a clinical observation of the course of the wound process and by those of planimetric and morphopathological studies.