Restoration of Bone Tissue in Femoral Condyles' Critical Defect in Rabbits Using Bioresorbable Calcium Carriers and Multipotent Mesenchymal Stromal Cells



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Abstract

Study of the efficacy of autologous bone marrow multipotent mesenchymal stromal cells (MMSC) with bioresorbable calcium carriers for the restoration of bone tissue in the site of critical distal femur spongy bone defect was performed in rabbits. System of rabbit's MMSC cultivation has been elaborated. After 2 - 3 passages MMSC were placed on bioresorbable calcium carriers and implanted into the site of preliminary created defect with 6 cm diameter. It was shown that implantation of MMSC in combination with bioresorbable carriers into the defect resulted in filling of spongy bone defect within 6 months after operation. The data obtained open promising perspectives for the application of MMSC in combination with appropriate carriers in tissue engineering for the treatment of vast skeleton injuries.

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