A new method for selective extraction of euglobals with monoterpenoid structure from Eucalyptus viminalis leaves


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Abstract

The purpose of this work is to test experimentally a working hypothesis about the possibility of euglobals' extraction from Eucalyptus viminalis leaves with two different perfluoro organic solvents, Novec 1230 and Novec 7100. Materials and methods. For extraction of euglobals from Eucalyptus viminalis leaves, we used a circulation method for extraction in Soxhlet extractor at plant raw material / solvent ratio 1:5 w/v. For analysis of euglobals, we used a method of reverse phase high performance liquid chromatography. As standard euglobal-containing substances, we used Spissum extract of Chlorophyllipt standard sample of the State Pharmacopoeia of Ukraine and eucalimin standard sample. Results. It is determined that perfluoro organic solvent Novec 1230 does not extract euglobals, while solvent Novec 7100 exhibits selective but incomplete extraction level of euglobals with monoterpenoid structure. A circulation method of solvent Novec 7100 in Soxhlet extractor demonstrates that in two hours of extraction from Eucalyptus viminalis leaves, the yield of dominant euglobal with monoterpenoid structure was equal to 49.3±2.5 %, and the yield of dry residue relatively to plant raw material initial weight was equal to 0.63±0.03 %. In the extract obtained, no chlorophylls or macrocarpales were detected. Conclusions. Our working hypothesis about the possibility of euglobals' extraction from Eucalyptus viminalis leaves with perfluoro organic solvent has been verified experimentally. The yield of euglobals significantly depend not only on the fluorination degree of the solvent molecule, but also on the value of topological polar surface area of euglobal molecule. A new method for selective extraction of euglobals with monoterpenoid structure from Eucalyptus viminalis leaves by perfluoro organic solvent Novec 7100 has been suggested.

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About the authors

N. N Boyko

Belgorod National Research University

Email: boykoniknik@gmail.com
Ph.D. (Pharm.), Junior Research Scientist of Laboratory Technology of Drugs, Associate Professor of Pharmaceutical Technology Department

E. T Zhilyakova

Belgorod National Research University

Dr.Sc. (Pharm.), Professor, Head of Pharmaceutical Technology Department

D. I Pisarev

"Drugs' Quality Control Center"; Peoples' Friendship University of Russia

Dr.Sc. (Pharm.), Professor of Shared Knowledge Center (Research and Educational Center)

O. O Novikov

"Drugs' Quality Control Center", Peoples' Friendship University of Russia

Dr.Sc. (Pharm.), Professor, Director of Shared Knowledge Center (Research and Educational Center)

P. G Mizina

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Dr.Sc. (Pharm.), Professor, Vice-Director for Science

O. L Saybel

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Ph.D. (Pharm.), Head of Chemistry and Pharmaceutical Technology Сenter

M. K Sidelnikova

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Ph.D. (Pharm.), Senior Research Scientist of Chemistry and Pharmaceutical Technology Сenter

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