Micromycetes collection strains study for search cultures with collagenolytic activity

Relevance. The collagenase producers search is of great interest for various areas of human activity. The study of various enzymes synthesized by microorganisms and the possibility of their practical application is one of the most relevant areas of biotechnology. These biologically active compounds are widely used in a number of the national economy sectors, in medical practice and also in research purposes. These enzymes include collagenases, which are the part of medicines used to remove scars, keloids and in the treatment of burns and ulcers. However, in domestic medical practice, mainly imported drugs are used for these purposes. Objective. The work is devoted to the search of these enzymes producers among the collection strains of mycelial fungi of All-Russian Scientific Research Institute of Medicinal and Aromatic Plants. Material and methods. It was conducted with using of surface cultivation on media with the replacement of sucrose on collagen. It is known that the growth of microorganisms on substrates containing insoluble proteins as the only source of carbon allows us to estimate their potential proteolytic activity. The appearance of protein lysis zones around colonies is an indicator of enzyme secretion into the environment. Results. The article shows that all studied mycelial fungi grew well on media with the replacement of easily metabolized carbohydrate on the collagen. During surface cultivation micromycetes formed lysis zones which indicates the synthesis and secretion of hydrolases into environment. Conclucion. In the result of a detailed study of 14 fungal strains, as well as regression and correlation analysis, 6 micromycetes promising for study as collagenase producers were selected.

micromycetes, collagen, collagenases