Studying morphogenic potential of annual clary (Salvia viridis L.) in vitro

Salvia viridis L. is widely cultivated as an ornamental plant, but it has medicinal value, including antioxidant, antibacterial and antifungal properties. The presence of flavonoids, caffeic acid derivatives and phenylethanoids may enhance its potential antioxidant properties. Plasmic seeds of S. viridis (PLAZMAS Ltd.) were used in the research. To obtain aseptic plant material, the seeds were sterilized with 5% sodium hypochlorite for 5, 10, and 15 minutes, then washed twice with distilled water. The seeds were placed on to phytohormone-free Murashige and Skoog (MS) nutrient medium. Petiole, leaf and stem expiants were cultivated on MS nutrient medium without NH4NO3 with the addition of various phytohormones and growth regulators: indolyl-3-acetic acid (IAA), a-naphthaleneacetic acid (NAA), indolyl-3-butyric acid (IBA), 6-benzylaminopurine (BAP); a phytohormone-free nutrient medium was used as a control. Based on the results of experiments, 5% sodium hypochlorite solution with holding time of 5 minutes can be recommended for surface sterilization of annual clary seeds. This mode provides the high yield of aseptic plants. The study of the morphogenic potential of S. viridis aseptic plants makes it possible to increase the multiplication factor of this valuable plant. It has been shown that stem and petiole expiants should be used for the induction of callusogenesis by cultivating expiants on MS nutrient medium with or without phytohormones and growth regulators. A high freguency of rhizogenesis can be obtained on petiole and stem expiants on MS nutrient medium with the addition of 1 mg/L IAA or NAA.

annual clary, in vitro culture, aseptic plants, callusogenesis, rhizogenesis