THE INFLUENCE OF COLD HELIUM PLASMA ON THE STATE OF FREE RADICAL PROCESSES UNDER EXPERIMENTAL THERMAL TRAUMA

The aim of the study was to estimate the effect of helium cold plasma (5 daily procedures of 1 min) on blood oxidative metabolism of rats with experimental thermal trauma. The experiment was performed on 30 male Wistar rats divided into 3 egual-sized groups. The first group of animals (n=10) was intact. The rats of the second and third groups (n=10 in each) were simulated contact thermal burn (area - 10% of the body surface) according to our own method, and local treatment of the wound with levomekol was performed. The animals of the third group were additionally treated with a course that included 5 daily procedures for treating a burn wound (1 min) localized on the skin of the back with a stream of cold helium plasma. The distance from the edge of the "plasma torch" to the treated surface was 1.0-1.2 cm. The cold plasma was produced using a special installation using the principle of microwave-induced ionization of the gas stream. Balloon helium of the A brand was used as the plasma carrier gas. The intensity of peroxidation in the plasma and membranes of red blood cells was studied by Fe-induced biochemiluminescence by the light sum of the latter. In addition, the analysis of the biochemiluminescence curve allowed us to calculate the total antioxidant activity of blood plasma. It was found that the course of treatment of an experimental burn wound with cold helium plasma has a positive effect on the blood metabolism of rats, allowing to neutralize the manifestations of oxidative stress and exerting an antioxidant effect. It was found that this effect is realized both in blood plasma and in red blood cell membranes.

cold plasma, blood, lipid peroxidation, thermal trauma