Investigation and validation of quantitative analysis of phenolic compounds and chlorogenic acid in the Vaccinium uliginosum leaves
- Authors: Shamilov A.A1, Bubenchikova V.N2, Garsiya E.R1, Ibaeva K.A1, Larsky M.V1
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Affiliations:
- Pyatigorsk Medical-Pharmaceutical Institute (PMPI), Branch of Volgograd State Medical University, Ministry of Health of Russia
- Kursk State Medical University, Ministry of Health of Russia
- Issue: Vol 25, No 2 (2022)
- Pages: 14-23
- Section: Articles
- URL: https://journals.eco-vector.com/1560-9596/article/view/112931
- DOI: https://doi.org/10.29296/25877313-2022-02-03
- ID: 112931
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Abstract
Relevance. The modern requirements of qualitative control of medicinal plant raw material recommends to evaluate total amount of biological active compounds and amount of major substances. Aim. Investigation and validation of quantitative analysis of total amount of phenolic compounds and major substance chlorogenic acid in the Vaccinium uliginosum leaves. Materials and methods. Quantitative analysis of total amount of phenolic compounds in the V. uliginosum leaves was carried out by spectrophotometry. Amount of chlorogenic acid was measured by capillary electrophoresis as main method and by high-performance liquid chromatography as comparative method. Developed methods were validated. Results. We investigated and validated methods of spectrophotometric analysis of total amount of phenolic compounds and quantitative analysis of chlorogenic acid by capillary electrophoresis. Total amount of phenolic compounds were 4.94±0.17% - 9,09±0,17%, amount of chlorogenic acid - 1.75±0.08% - 4.34±0.16% Conclusions. Medicinal plant raw material - Vaccinium uliginosum leaves -are recommended to be standardized by total amount of phenolic compounds and amount of major substance - chlorogenic acid - using spectrophotometry and capillary electrophoresis respectively. Total amount of phenolic compounds calculated to g chlorogenic acid at 100 g of raw material should be no less 4.5%, amount of chlorogenic acid - no less 1.5%.
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About the authors
A. A Shamilov
Pyatigorsk Medical-Pharmaceutical Institute (PMPI), Branch of Volgograd State Medical University, Ministry of Health of Russia
Author for correspondence.
Email: shamilovxii@yandex.ru
Ph.D. (Pharm.), Associate Professor of Department of Pharmacognosy, Botany and Technology of Phytopreparations Pyatigorsk, Russia
V. N Bubenchikova
Kursk State Medical University, Ministry of Health of Russia
Email: bubenhikova.ksmu@yandex.ru
Dr.Sc. (Pharm.), Professor, Head of Department of Pharmacognosy and Botany Kursk, Russia
E. R Garsiya
Pyatigorsk Medical-Pharmaceutical Institute (PMPI), Branch of Volgograd State Medical University, Ministry of Health of Russia
Email: x-pharm@mail.ru
Lecturer, Department of Pharmacognosy, Botany and Technology of Phytopreparations, Department of Inorganic, Physical and Colloidal Chemistry Pyatigorsk, Russia
Kh. A Ibaeva
Pyatigorsk Medical-Pharmaceutical Institute (PMPI), Branch of Volgograd State Medical University, Ministry of Health of Russia
Email: ibaeva.hadizhat@mail.ru
Post-graduate Student, Department of Pharmacognosy, Botany and Technology of Phytopreparations Pyatigorsk, Russia
M. V Larsky
Pyatigorsk Medical-Pharmaceutical Institute (PMPI), Branch of Volgograd State Medical University, Ministry of Health of Russia
Email: m.v.larsky@pmedpharm.ru
Ph.D. (Pharm.), Head of Department of Pharmaceutical Chemistry Pyatigorsk, Russia
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