Development of an identification method vaccinium uliginosum leaves by thin-layer chromatography

A.A. ShamilovPh.D. (Pharm.), Associate Professor, Department of Pharmacognosy, Botany and Technology of Phytopreparations, Pyatigorsk Medical-Pharmaceutical Institute, Branch of Volgograd State Medical University (Pyatigorsk, Russia)E-mail: shamilovxii@yandex.ru; ORCID iD 0000-0002-6730-9518V.N. BubenchikovaDr.Sc. (Pharm.), Professor, Head of Department of Pharmacognosy and Botany, Kursk State Medical University (Kursk, Russia)E-mail: bubenhikova.ksmu@yandex.ru;ORCID iD0000-0001-9682-0684E.R. GarsiyaLecturer, Department of Pharmacognosy, Botany and Technology of Phytopreparations and Department of Inorganic, Physical and Colloidal Chemistry,Pyatigorsk Medical-Pharmaceutical Institute, Branch of Volgograd State Medical University (Pyatigorsk, Russia) E-mail: x-pharm@mail.ru; ORCID iD 0000-0003-3217-0680Kh. A. IbaevaPost-graduate Student, Department of Pharmacognosy, Botany and Technology of Phytopreparations, Pyatigorsk Medical-Pharmaceutical Institute, Branch of Volgograd State Medical University (Pyatigorsk, Russia)E-mail: ibaeva.hadizhat@mail.ruM.V. LarskyPh.D. (Pharm.), Head of Department of Pharmaceutical Chemistry, Pyatigorsk Medical-Pharmaceutical Institute, Branch of Volgograd State Medical University (Pyatigorsk, Russia)E-mail: m.v.larsky@pmedpharm.ru; ORCID iD0000-0002-4406-7165

Relevance. Development of new methods and improvement of existing methods of identification and quality control new and pharmacopoeia plant raw material involve detection of major or marker component. End-to-end standardization of plant raw material and drugs from these plant material can be carried out on this component.

Aim. Development of qualitative control analysis of Vaccinium uliginosum leaves by marker component – chlorogenic acid – using thin-layer chromatography.

Material and methods. Qualitative control analysis of V. uliginosum leaves was carried out with water-alcohol extract (ethanol 70%) from air-dried leaves. We have selected the best conditions for separating the marker component – chlorogenic acid – from other compounds: mobile phase, detection, application, type of chromatographic plate.

Results. Determination of the main group of biologically active compounds in the V. uliginosum leaves by thin-layer chromatography can be carried out under the following conditions:sorbent – silica gel, polymer or aluminum plates, eluent – anhydrous formic acid-glacial acetic acid–water–ethylacetate (7.5:7.5:18:67), detector – ammonia and aluminum chloride alcohol solution 2%, injection volume 7.5 µl of water–ethanol extract, 5 µl of chlorogenic acid standard solution, time of saturation of the chamber with eluent vapors – 30 min, elution time – 55–60 min, storing time in the dish drying cabinet after treat with a aluminum chloride alcohol solution2% 3 min.

Conclusions. We have developed qualitative control method of plant raw material – Vaccinium uliginosum leaves–by marker component chlorogenic acid using thin-layer chromatography.