Blood xanthine oxidoreductase: activity profile in systemic lupuse erythematosus
- Authors: Mozgovaya E.E.1, Bedina S.A.1, Trofimenko A.S.1, Spitsina S.S.1, Mamus M.A.1, Zborovskaya I.A.1
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Affiliations:
- Research Institute of Clinical and Experimental Rheumatology named after A.B. Zborovsky
- Issue: Vol 25, No 11 (2022)
- Pages: 36-41
- Section: Articles
- URL: https://journals.eco-vector.com/1560-9596/article/view/113008
- DOI: https://doi.org/10.29296/25877313-2022-11-06
- ID: 113008
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Abstract
Relevance. Oxidative stress is an integral part of the pathogenesis of systemic lupus erythematosus (SLE). At the same time, hyperproduction of reactive oxygen and nitrogen species prevails over the capabilities of the antioxidant system. Xanthine oxidoreductase (XOR) is an important source of reactive oxygen species, reactive nitrogen species, and nitric oxide. The enzyme exhibits its activity in interconvertible forms: xanthine oxidase (XO; EC 1.17.3.2) and xanthine dehydrogenase (XDG; EC 1.17.1.4).
Objective – analysis of XDH and XO activities profiles in blood depending on the activity of SLE.
Material and methods. 56 SLE (diagnosis of SLE was verified using the EULAR/ACR criteria (2019)) patients and 35 healthy controls were enrolled in this study. Patients with SLE were divided into 3 subgroups, according to the degree of process activity (classification based on clinical manifestations and the SLEDAI 2K index were used). Enzymes activities levels were measured in plasma (KOpl, XDGpl), lysed WBC (KOl, XDGl), and lysed RBC (KOer, XDGer).
Results. The enzyme indices of SLE patients significantly differed from the control values both in blood plasma and in cell lysates. Relationships between the activity levels of the D-form, as well as the O-form of XOR and the SLEDAI 2K index were revealed. The increase in the levels of XOpl, XDGer and a decrease in the levels of XDGpl, XOl, XDGl, XOer were determined on the background of an increase in disease activity. The levels of XO and XDG in plasma and cell lysates differed significantly between subgroups of patients with different SLE activity.
Conclusion. The significant changes in the XOR enzyme system, which depend on the activity of immune inflammation, were revealed in SLE. Changes in the balance of O- and D-forms XOR levels in plasma, lysed WBC and lysed RBC determine the blood profiles characteristic of different activity degrees in SLE.
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About the authors
E. E. Mozgovaya
Research Institute of Clinical and Experimental Rheumatology named after A.B. Zborovsky
Author for correspondence.
Email: nauka@pebma.org
Ph.D. (Med.)
Russian Federation, VolgogradS. A. Bedina
Research Institute of Clinical and Experimental Rheumatology named after A.B. Zborovsky
Email: nauka@pebma.org
Ph.D. (Med.)
Russian Federation, VolgogradA. S. Trofimenko
Research Institute of Clinical and Experimental Rheumatology named after A.B. Zborovsky
Email: nauka@pebma.org
Ph.D. (Med.)
Russian Federation, VolgogradS. S. Spitsina
Research Institute of Clinical and Experimental Rheumatology named after A.B. Zborovsky
Email: nauka@pebma.org
Junior Researcher
Russian Federation, VolgogradM. A. Mamus
Research Institute of Clinical and Experimental Rheumatology named after A.B. Zborovsky
Email: nauka@pebma.org
Junior Researcher
Russian Federation, VolgogradI. A. Zborovskaya
Research Institute of Clinical and Experimental Rheumatology named after A.B. Zborovsky
Email: nauka@pebma.org
Dr.Sc. (Med.), Professor
Russian Federation, VolgogradReferences
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