Carotenoids and chlorophylls when present together: analytical and technological features

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Abstract

Relevance.An analytical approach to choosing the direction of technological research has always been relevant, and taking into account the production of drugs using “green” extractants, the justification of technological parameters of extraction and its analytical support on models of already known, widely studied and used plant objects, for example, stinging nettle (Urtica dioica L.), can be considered as rational.

The purpose of the study is an analytical study of carotenoids and chlorophylls in the co-presence of stinging nettle leaves and the rationale for the temperature regime for obtaining an oil extract from this plant, taking into account the co-presence of pigments in the extracts.

Material and methods. The content of carotenoids and chlorophylls in raw materials and oil extracts was determined by spectrophotometry. Oil extracts were obtained by fractional maceration at two temperature conditions: 25 °C and 50 °C.

Results. The content of carotenoids and chlorophylls when jointly present in stinging nettle leaves was determined by spectrophotometry. When obtaining six model oil extracts of stinging nettle leaves, the temperature regime was experimentally optimized at 50 °C; the optimization parameter was the quantitative content of carotenoids and chlorophylls.

Conclusion. A detailed determination of the content of chlorophyll a, chlorophyll b, their sum and carotenoids in the presence of chlorophylls gives a detailed idea of the quantitative ratio of these types of pigments in the raw material and makes it possible to eliminate the overlap of absorption bands of carotenoids and chlorophylls. This approach made it possible to optimize the temperature conditions for obtaining the oil extract of nettle leaves. The applied analytical approach can be used to control the content of carotenoids and chlorophylls when present together to optimize the technological parameters of oil extracts obtained from other types of medicinal plant raw materials.

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About the authors

A. G. Kuregyan

Pyatigorsk Medical Pharmaceutical Institute – Branch of Volgograd State Medical University

Author for correspondence.
Email: Kooreguan@mail.ru
ORCID iD: 0000-0002-0698-8254
SPIN-code: 4547-1787
Scopus Author ID: 6506046751

Dr.Sc. (Pharm.), Professor, Department of Pharmaceutical Chemistry

Russian Federation, Pyatigorsk

S. V. Pechinskii

Pyatigorsk Medical Pharmaceutical Institute – Branch of Volgograd State Medical University

Email: hplc@yandex.ru
ORCID iD: 0000-0002-9505-9990
SPIN-code: 9798-4663
Scopus Author ID: 55993869200

Ph.D (Pharm.), Associate Professor, Department of Pharmaceutical Chemistry

Russian Federation, Pyatigorsk

A. A. Markaryan

Moscow State Medical and Dental University named after. A.I. Evdokimova of the Ministry of Health of Russia

Email: Kooreguan@mail.ru

Dr.Sc. (Pharm.), Vice-Rector

Russian Federation, Moscow

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2. Рис. 1. Типичный спектр поглощения спиртового экстракта из крапивы двудомной листьев (серия 1)

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3. Rice. 2. Typical absorption spectra of oil extracts of stinging nettle leaves obtained at temperatures of 20 °C (a) and 50 °C (b) in 100% acetone

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4. Rice. 3. Typical absorption spectra of oil extracts of nettle leaves obtained at temperatures of 20 °C (a) and 50 °C (b) in diethyl ether

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