Trace element composition of raw materials taken from genus Pulmonaria plants to substantiate its' antianemic action

Introduction. Total extracts taken from generative shoots of genus Pulmonaria plants have antianemic activity. At the same time, extracts obtained from vegetative leaves of these plants do not have similar action. Nowadays, there is no algorithm for determining authenticity and quality of herb raw material with using biogenic elements. Creating such algorithm for diagnosing raw materials by microelement composition is an urgent task.

Materials and methods. The research objects were generative shoots and vegetative leaves of such plants as: P. officinalis L., P. obscura Dum. and P. mollis Wulf. ex Horn. and mixtures of both types of raw materials. Quantitative determination of elements was carried out by mass spectrometry with inductively coupled plasma. Analyzing obtained results was made using the method of hierarchical clustering.

Results. It was established the division into clusters considering all trace elements confirm the species-specificity of the trace element status plants. At the same time raw materials with impurities form clusters different from the cluster formed by hematopoietic complex trace elements contained in the generative shoots of the studied plants. The discovered regularity allows us to consider the cluster, which included the generative shoots as a reference cluster. This reference cluster makes it possible to form an algorithm for checking the raw materials, and its quality

Conclusions. As a result of the work, the possibility of using the trace element composition of plants for the standardizing herb raw materials was established. In addition, the algorithm has been proposed that allows using the content of hematopoietic complex trace elements to determine the authenticity and quality of the herb raw material "Pulmonaria herba".