Optimization of the conditions of ultrasonic extraction of polyphenolic compounds from the inflorescences of Koenigia weyrichii (F. Schmidt) T.M. Schust. Et Reveal

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Abstract

Introduction. Inflorescences of Koenigia weyrichii (F. Schmidt) T.M. Schust. et Reveal contains a large amount of flavonoids. However, at present, the most optimal conditions for their extraction by water-ethanol mixtures have not been obtained, and the effect of ultrasound on the extraction process has not been fully studied.

The aim of the study was to optimize the conditions of ultrasonic water-ethanol extraction of secondary metabolites from the inflorescences of K. weyrichii.

Material and methods. Optimal extraction conditions have been established using the Box-Behnken algorithm. The total content of polyphenols was determined using the Folin-Ciocalteu method, and flavonoids were determined using a complexation reaction with aluminum chloride. The total antioxidant activity was assessed using the phosphomolybdate method. The content of polyphenolic acids was determined by HPLC. The ability of the extract obtained under optimal conditions to inhibit free radicals was determined by DPPH and ABTS methods.

Results. The highest yield of the target substances is achieved in 60 minutes of extraction in 50% aqueous ethanol at a temperature of 50 ° C, and mixing plant material with a particle size of 0.1 mm with an solvent in a ratio of 1:10 (mass to volume. It has been shown that ultrasound exposure reduces the yield of target substances.

Conclusions. The results of the work are the scientific basis for the development of methods of phytochemical analysis and technologies for the production of pharmaceuticals based on the studied plant.

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About the authors

A. V. Korovkina

Federal Research Centre “Kola Science Centre of the Russian Academy of Sciences”

Author for correspondence.
Email: a.korovkina@ksc.ru
ORCID iD: 0000-0002-4097-6521

Research Scientist, Laboratory of Medical and Biological Technologies

Russian Federation, Fersmana str. 14, Apatity, 184209

A. A. Koigerova

Federal Research Centre “Kola Science Centre of the Russian Academy of Sciences”

Email: a.koygerova@ksc.ru
ORCID iD: 0000-0002-8843-0122

Research Scientist, Laboratory of Medical and Biological Technologies

Russian Federation, Fersmana str. 14, Apatity, 184209

N. S. Tsvetov

Federal Research Centre “Kola Science Centre of the Russian Academy of Sciences”

Email: n.tsvetov@ksc.ru
ORCID iD: 0000-0003-1356-2259

Ph.D. (Chem.), Head of the Laboratory of Medical and Biological Technologies, Scientific Center for Medical and Biological Research of Human Adaptation in the Arctic

Russian Federation, Fersmana str. 14, Apatity, 184209

P. G. Mizina

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Email: mizina-pg@yandex.ru
ORCID iD: 0000-0001-6510-9603

Dr.Sc. (Pharm.), Professor, Advisor to the Director, Honored Scientist of the Russian Federation

Russian Federation, Grin str. 7, Moscow, 117216

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Supplementary files

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1. JATS XML
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2. Fig. 1. Dependences of the yield of polyphenols (а), flavonoids (б) and total antioxidant activity (в) on extraction time and approximation results (—)

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3. Fig. 2. The effect of ethanol concentration on the yield of the sum of polyphenols TPC (а), flavonoids TFC (б) and total antioxidant activity TAC (в)

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4. Fig. 3. Dependence of TPC (а), TFC (б) and TAC (в) on the power of ultrasonic exposure P

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5. Fig. 4. Contour-plots of the response surfaces for the total content of polyphenols TPC (а–в), flavonoids TFC (г–е) and total antioxidant activity TAC (ж–и) from temperature T (C), the ratio of the volume of solvent and the mass of plant raw materials v/m (B) and particle size of vegetable raw materials d (C)

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6. Fig. 5. Experimental data on the dependence of the degree of inhibition of free radicals DPPH (а) and ABTS (б), as well as the results of data approximation (---) by a linear equation in the case of the DPPH radical and the Boltzmann sigmoid in the case of the ABTS radical

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