Comparative study of the content of biologically active substances in the herba and leaves of Bupleurum aureum (Bupleurum aureum Fisch. seu longifolium L.)

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Abstract

Intriduction. Bupleurum aureum Fisch. seu longifolium L. is a perennial plant of practical interest for pharmacy due to its hepatoprotective and anti-inflammatory activity. In this study, the problems of standardization of raw materials related to the heterogeneity of harvested grass were studied, and a hypothesis was put forward about the predominant accumulation of flavonoids in the leaves of the plant.

The purpose of the study is to develop a technologically sound method for extracting flavonoids from the raw materials of golden malt based on a comparative analysis of the content of BAS in the aboveground part of the plant.

Material and methods. In this work we used raw material of the herb of the Bupleurum, collected in the Altai Territory by the company “Horst”. Quantitative determination of the total amount of flavonoids in terms of rutin was carried out by differential spectrophotometry.

Results. As a result of the study was developed a technique for the quantitative determination of the total amount of flavonoids in terms of rutin in the leaves of the Bupleurum, for the developed technique also determined metrological characteristics. According to the obtained data, the total amount of flavonoids in the leaves of the Bupleurum is within 6.45±0.18% in comparison with the content of flavonoids 2.91± 0.17%, obtained for the herb of the Bupleurum.

Conclusion. Thus, the removal of stems from the collected raw material allows to increase the yield of flavonoids per kg of raw material in 2.2 times, which indicates the feasibility of using leaves of the Bupleurum as a raw material for extracts.

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About the authors

V. S. Bobyleva

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Author for correspondence.
Email: vitaliya.bobyleva@yandex.ru
ORCID iD: 0009-0005-0149-4772
SPIN-code: 7013-8640

Junior Research Scientist, Experimental-technological Department

Russian Federation, 7 Green str., Moscow, 117216

М. A. Dzhavakhyan

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants; Russian University of Medicine of the Ministry of Health of the Russian Federation

Email: akopovamarina13@mail.ru
ORCID iD: 0000-0003-2673-6203
SPIN-code: 3912-4027

Dr.Sc. (Pharm.), Chief Scientific Officer, Experimental-technological Department, Deputy Director for Development and Implementation

Russian Federation, 7 Green str., Moscow, 117216; Delegatskaya Street, 20/1, Moscow, 127473

V. N. Dul

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants; Russian University of Medicine of the Ministry of Health of the Russian Federation

Email: dvnslava@rambler.ru
ORCID iD: 0000-0001-7824-4417
SPIN-code: 8192-5803

Ph.D. (Pharm.), Leading Research Scientist, Testing Center, Research Scientist, Research Laboratory for the Development and Implementation of Innovative Drugs

Russian Federation, 7 Green str., Moscow, 117216; Delegatskaya Street, 20/1, Moscow, 127473

O. A. Semkina

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Email: semkina@vilarnii.ru
ORCID iD: 0000-0002-2611-4490
SPIN-code: 1637-9398

Ph.D. (Pharm.), Leading Research Scientist, Experimental-technological Department

Russian Federation, 7 Green str., Moscow, 117216

T. V. Kachalina

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Email: Kachalina_tat@bk.ru
ORCID iD: 0000-0002-3459-5335
SPIN-code: 4334-9912

Ph.D. (Pharm.), Leading Research Scientist, Experimental-technological Department

Russian Federation, 7 Green str., Moscow, 117216

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Supplementary files

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2. Fig. 1. Primary differential UV spectra of a complex of standard sample of rutin and AlCl3 and the extracts from the leaves, herba and stems of the Bupleurum aureum (λmax= 410±2 nm)

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3. Fig. 2. The effect of the type of extractant on the yield of flavonoids into extract obtained from the leaves of the Bupleurum aureum (n = 3)

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4. Fig. 3. Dependence of the determined content of flavonoids in terms of rutin in the extract of leaves of Bupleurum aureum on the amount of added 2% AlCl3 (n = 3)

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