Influence of Calendula officinalis L. extract on the development of experimental hepatitis

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Abstract

The purpose of the study was to evaluate the effect of dry extract of Calendula officinalis L. on the course of experimental hepatitis caused by the administration of carbon tetrachloride in male Wistar rats.

Material and methods. The experimental hepatitis in Wistar rats was caused by four subcutaneous injections of a 50% oil solution of carbon tetrachloride at a dose of 4 ml/kg. The dry extract C. officinalis was administered intragastrically at a dose of 100 mg/kg to animals from the second day of the experiment for 20 days. The choleretic function of the liver was assessed in Wistar rats on 7th, 14th and 21th day. The intensity of oxidative stress, the state of the antioxidant system, the energy metabolism of hepatocytes and pathomorphological studies of the liver were held on 14th day.

Results. It has been established that C. officinalis dry extract in an experimental therapeutic dose has a beneficial effect on the course of acute toxic hepatitis in rats, reduced the intensity of free radical oxidation, limited the inflammatory reaction, reduced the severity of the main manifestations of liver damage – cytolysis, cholestasis, and also corrected energy processes in hepatocytes. Pathomorphological studies confirmed the hepatoprotective effect of the extract C. officinalis.

Conclusion. The dry extract C. officinalis at a dose of 100 mg/kg has a hepatoprotective effect in experimental toxic hepatitis.

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About the authors

А. А. Toropova

Institute of General and Experimental Biology

Author for correspondence.
Email: anyuta-tor@mail.ru
ORCID iD: 0000-0003-2618-7777

Ph.D. (Biol.), Senior Research Scientist of Laboratory of Bioactive Compounds Safety

Russian Federation, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

Z. G. Sambueva

Institute of General and Experimental Biology

Email: zinasamb@yandex.ru
ORCID iD: 0009-0009-2155-5789

Ph.D. (Biol.), Senior Research Scientist of Laboratory of Bioactive Compounds Safety

Russian Federation, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

Ya. G. Razuvaeva

Institute of General and Experimental Biology

Email: tatur75@mail.ru
ORCID iD: 0000-0001-7829-1424

Dr. (Biol.), Senior Research Scientist of Laboratory of Bioactive Compounds Safety

Russian Federation, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

D. N. Olennikov

Institute of General and Experimental Biology

Email: olennikovdn@mail.ru
ORCID iD: 0000-0001-8194-1061

Dr. (Pharm.), Head of the Laboratory of Biomedical Research

Réunion, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

N. S. Badmaev

Institute of General and Experimental Biology

Email: nikita.bad@list.ru
ORCID iD: 0009-0009-9026-0992

Post-graduate Student of Laboratory of Experimental Pharmacology

Russian Federation, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

S. М. Nikolaev

Institute of General and Experimental Biology

Email: smnikolaev@mail.ru
ORCID iD: 0000-0002-5833-7576

Dr. (Med), Professor, Chief Researcher of Laboratory of Experimental Pharmacology

Russian Federation, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

V. Е. Khitricheyev

Buryat State University

Email: khitrikheev-ve@yandex.ru
ORCID iD: 0000-0002-4400-8085

Dr. (Med), Professor, Head of the Department of Hospital Surgery Medical Institute

Russian Federation, 670000, Ulan-Ude, Smolina Str., 24A

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2. Fig. 1. Effect of C. officinalis extract on the state of pro- and antioxidant systems in the liver in Wistar rats with CCl4-hepatitis (14th day): GPO – glutathione peroxidase, RG – reduced glutathione, GR – glutathione reductase, MDA – malondialdehyde

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3. Fig. 2. The effect of C. officinalis extract on the energy status of the liver in Wistar rats with CCl4-hepatitis (14th day): MK – lactic acid, PVC – pyruvic acid, ATP – adenosine triphosphoric acid

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